【摘要】 为了解垃圾渗滤液处理系统中菌群结构与脱氨效果的关系,采用16S rRNA基因高通量测序技术分析了15个实际垃圾渗滤液处理系统中的自养氨氧化细菌(AOB)的群落结构,监测了其中1个处理系统中的AOB群落结构的周年变化,并采集实际垃圾渗滤液开展了为期45d的模拟试验.结果表明,Nitrosomonas属是垃圾渗滤液处理系统中的优势自养氨氧化细菌,其相对丰度与脱氨活性呈显著正相关(P<0.05).在此菌属中,N.europaea、N.eutropha和N. halophila的相对丰度与脱氨活性显著正相关(P<0.05),这3种菌在系统发育上同属于N. europaea/Nitrosococcus mobilis谱系,说明该谱系是实际垃圾渗沥液处理系统中的优势AOB类群,并对垃圾渗滤液脱氨有重要贡献.更多还原

【Abstract】 In order to study the relationship between the microbial community structure and the deamination effect in landfill leachate treatment systems, 16 S rRNA high-throughput sequencing technology was used to analyze the community structure of autotrophic ammonia oxidizing bacteria(AOB) in 15 full-scale landfill leachate treatment systems. Meanwhile, the annual change of the community structure of AOB in one of the treatment systems was monitored. Moreover, a 45-day simulation test using real landfill leachate was carried out to study the deamination activity. The results showed that Nitrosomonas was the dominant AOB genus in landfill leachate treatment systems, and its relative abundance was positively correlated with the deamination activity(P<0.05). Within the genus, the relative abundance of N. europaea, N. eutropha and N. halophila, which all belongs to the N. europaea/Nitrosococcus mobilis lineage, were positively correlated with deamination activity(P<0.05). The results indicated that the N. europaea/Nitrosococcus mobilis lineage was the dominant AOB group in the full-scale landfill leachate treatment systems, and greatly contributed to the deamination in landfill leachate.更多还原