【摘要】 为掌握广西地区导致猪群腹泻的主要病毒性病原的流行态势,为养殖场猪病毒性腹泻的综合防控提供依据,用实时荧光RT-PCR检测方法,对2017年1月—2021年6月广西14个地市489个猪场1 206份临床腹泻样品进行猪流行性腹泻病毒(PEDV)、猪轮状病毒(PoRV)、猪传染性胃肠炎病毒(TGEV)、猪丁型冠状病毒(PDCoV)等病原检测,并分析其阳性检出率在不同年份、季节、地区、年龄段猪群之间的差异以及病原混合感染情况。结果显示,2017—2021年,PEDV、PDCoV、PoRV、TGEV样品阳性检出率分别为49.67%、7.63%、4.56%和0.33%,场阳性检出率分别为53.37%、8.18%、8.18%和0.61%;病毒性腹泻病原有一定的季节性流行特征,多表现为冬、春季流行加重;PEDV、PDCoV普遍流行,PoRV局部流行,TGEV散发流行;4种病原对不同年龄段腹泻猪群的感染率存在差异,其中对哺乳仔猪危害最为严重;猪群以单一感染为主,主要为PEDV单一感染,阳性检出率为47.93%,混合感染均为二重感染且感染率较低。结果表明,导致广西地区猪群腹泻的主要病毒性病原主要为PEDV、PDCoV和PoRV,其中PEDV为最主要病原,其他病原也呈流行加重趋势,需进一步加强病原监测和疫病综合防控。更多还原

【Abstract】 In order to learn the prevalence of major viral pathogens leading to porcine diarrhea in Guangxi,and to provide a basis for comprehensive prevention and control of the disease in swine farms, 1 206 clinical diarrhea samples collected from 489 farms in 14 cities/regions in the province during January 2017 to June 2021 were detected for major pathogens including porcine epidemic diarrhea virus(PEDV),porcine rotavirus(PoRV),transmissible gastroenteritis virus(TGEV) and porcine coronavirus(PDCoV),and the differences of their positive detection rates in terms of different years,seasons, regions and pig populations at different ages as well as mixed infection with pathogens were analyzed.The results showed that the average positive detection rates of the above pathogens were49.67%,7.63%,4.56% and 0.33%,respectively,the positive rates at farm level were 53.37%,8.18%,8.18% and0.61%, respectively;viral diarrhea pathogens were characterized by certain seasonal prevalence,which were serious in winter and spring;PEDV and PDCoV were widely distributed,while PoRV was local and TGEV was sporadic;the infection rates of the four pathogens were different in diarrheal pigs at different ages,especially in suckling pigs that were hazarded to largest extent;pig populations were mainly infected with PEDV only,with the positive rate of47.93%,and all mixed infection was dual with relatively low infection rate.It was concluded that the pathogens leading to viral diarrhea in pigs mainly included PEDV,PDCoV and PoRV,especially PEDV,and other pathogens trended to be increasingly prevalent,which should be further monitored,prevented and controlled in the future.更多还原