【摘要】 目的探讨子宫内膜容受性（Endometrial receptivity,ER）标志物整合素β₁、整合素β₃、Rac1、HoxA11、Noggin在增生期及种植窗期的表达及与反复种植失败（Repeated implantation failure,RIF）的相关性。方法收集淮安市妇幼保健院2018年1月～2020年12月行体外受精-胚胎移植（In vitro fertilization-embryo transfer,IVF-ET）治疗的140例患者的增生期及种植窗期内膜组织。采用酶联免疫吸附（Enzyme linked immunosorbent assay,ELISA）法检测RIF患者（RIF组,n=80）和成功妊娠患者（对照组,n=60）整合素β₁、整合素β₃、Rac1、HoxA11、Noggin在增生期及种植窗期的表达水平。采用Spearman相关性分析种植窗期整合素β₁、整合素β₃、Rac1、HoxA11、Noggin与RIF的关系。结果增生期,RIF组ER标志物整合素β₁水平显著高于对照组（P <0.05）,Noggin水平显著低于对照组（P <0.05）。种植窗期,RIF组ER标志物整合素β₁水平显著高于对照组（P <0.05）,整合素β₃、Rac1、HoxA11、Noggin水平显著低于对照组（P <0.05）。种植窗期各组ER标志物整合素β₁、整合素β₃、Rac1、HoxA11、Noggin水平均显著高于增生期（P <0.05）。Spearman相关性分析结果显示,种植窗期整合素β₁水平与RIF呈正相关（r=0.615,P=0.021）,整合素β₃、HoxA11水平与RIF呈负相关（r=-0.599,P=0.032;r=-0.732,P=0.013）,Rac1、Noggin水平与RIF无相关性（r=-0.412,P=0.063;r=-0.357,P=0.081）。结论整合素β₁、整合素β₃、Rac1、HoxA11、Noggin在增生期及种植窗期均有表达。种植窗期子宫内膜整合素β₁表达水平升高和整合素β₃、HoxA11表达水平降低与RIF发生密切相关。更多还原

【Abstract】 Objective To investigate the expression of ER markers integrin β₁, integrin β₃, Rac1, Hox A11 and Noggin in proliferative stage and implantation window stage and their correlation with RIF. Methods 140 cases of IVF-ET endometrial tissues in proliferative stage and implantation window stage were collected from Huai’an maternal and child health hospital from Jan 2018 to Dec 2020. The expression levels of integrin β₁, integrin β₃, Rac1, HoxA11 and Noggin in patients with RIF（RIF group, n = 80） and patients with successful pregnancy（control group, n = 60） were detected by enzyme linked immunosorbent assay（ELISA）.Spearman correlation was used to analyze the relationship between integrin β₁, integrin β₃, Rac1, HoxA11, Noggin and RIF. Results In proliferative stage, the level of ER marker integrin β₁ in RIF group was significantly higher than that in the control group（P < 0.05）, and the level of Noggin in RIF group was significantly lower than that in the control group（P < 0.05）. At the implantation window stage, the level of ER marker integrin β₁ in RIF group was significantly higher than that in the control group（P < 0.05）, and the levels of integrin β₃, Rac1, HoxA11 and Noggin in RIF group were significantly lower than those in the control group（P< 0.05）. The levels of ER markers integrin β₁, integrin β₃, Rac1, HoxA11 and Noggin in the implantation window stage were significantly higher than those in the proliferative stage（P < 0.05）. Spearman correlation analysis showed that integrin β₁ level was positively correlated with RIF（r = 0.615, P = 0.021）, integrin β₃ and HOXA11 levels were negatively correlated with RIF（r =-0.599, P = 0.032; r =-0.732, P = 0.013）, Rac1 and Noggin levels were not correlated with RIF（r =-0.412, P = 0.063; r =-0.357, P = 0.081）. Conclusion Integrin β₁, integrin β₃, Rac1, HoxA11 and Noggin are expressed in proliferative stage and planting window stage. The increase of integrin β₁ and the decrease of integrin β₃ and HOXA11 in the implantation window are closely related to RIF.更多还原