【摘要】 目的:探讨莪术醇对转化生长因子β（TGF-β）诱导的子宫内膜癌RL-95细胞生长及纤维化相关蛋白表达的影响,并探讨其机制是否与调控微小RNA-214（miR-214）表达有关。方法:以TGF-β诱导RL-95细胞纤维化作为子宫内膜异位症细胞模型。设置对照（control）组、TGF-β组、TGF-β+低剂量莪术醇组、TGF-β+中剂量莪术醇组、TGF-β+高剂量莪术醇组、TGF-β+高剂量莪术醇+anti-miR-NC组和TGF-β+高剂量莪术醇+anti-miR-214组。运用CCK-8法、流式细胞术、RT-qPCR和Western blot检测细胞活力、细胞周期分布、miR-214表达以及α-平滑肌肌动蛋白（α-SMA）和Ⅰ型胶原（Col I）蛋白表达。结果:与control组比较,TGF-β组RL-95细胞活力、S期细胞比例及α-SMA和Col I蛋白表达显著升高,G₀-G₁期细胞比例和miR-214表达显著降低（P<0.05）。与TGF-β组比较,TGF-β+中剂量莪术醇组和TGF-β+高剂量莪术醇组RL-95细胞活力、S期细胞比例及α-SMA和Col I蛋白表达显著降低,G₀-G₁期细胞比例和miR-214表达显著升高（P<0.05）。与TGF-β+高剂量莪术醇+anti-miR-NC组比较,TGF-β+高剂量莪术醇+anti-miR-214组RL-95细胞活力、S期细胞比例及α-SMA和Col I蛋白表达显著升高,G₀-G₁期细胞比例显著降低（P<0.05）。结论:莪术醇通过上调miR-214表达抑制TGF-β诱导的RL-95细胞生长和纤维化相关蛋白表达,进而对子宫内膜异位症纤维化具有潜在治疗作用。更多还原

【Abstract】 AIM:To investigate the effect of curcumol on the growth and fibrosis-related protein expression in endometrial adenosquamous carcinoma RL-95 cells induced by transforming growth factor-β（TGF-β）,and to explore whether the mechanism is related to the regulation of microRNA-214（miR-214）expression.METHODS:The RL-95 cells induced by TGF-β were used to mimic an in vitro endometrial fibrosis model,and the cells were divided into control group,TGF-β group,TGF-β+low-dose curcumol group,TGF-β+medium-dose curcumol group,TGF-β+high-dose curcumol group,TGF-β+high-dose curcumol+anti-miR-NC group and TGF-β+high-dose curcumol+anti-miR-214 group. The CCK-8 assay,flow cytometry,RT-qPCR and Western blot were applied to detect cell viability,cell cycle distribution,miR-214 expression and the protein expression of α-smooth muscle actin（α-SMA）and collagen type I（Col I）.RESULTS:Compared with control group,the cell viability,S-phase cell proportion,and the protein expression of α-SMA and Col I in the RL-95 cells in TGF-β group were significantly increased,while G₀/G₁-phase cell proportion and miR-214 expression were significantly reduced（P<0. 05）. Compared with TGF-β group,the cell viability,S-phase cell proportion,and the protein expression of α-SMA and Col I in the RL-95 cells in TGF-β+medium-dose curcumol group and TGF-β+high-dose curcumol group were significantly reduced,while G₀/G₁-phase cell proportion and miR-214 expression were significantly increased（P<0. 05）. Compared with TGF-β+high-dose curcumol+anti-miR-NC group,the cell viability,Sphase cell proportion,and the protein expression of α-SMA and Col I in the RL-95 cells in TGF-β+high-dose curcumol+anti-miR-214 group were significantly increased,while G₀/G₁-phase cell proportion was significantly reduced（P<0. 05）.CONCLUSION:Curcumol inhibits TGF-β-induced RL-95 cell growth and fibrosis-related protein expression by up-regulating miR-214 expression,thus having a potential therapeutic effect on endometriosis fibrosis.更多还原