【摘要】 目的研究大蒜素对胃癌细胞上皮-间质转化（EMT）的抑制作用及其机制。方法以含10%胎牛血清的RPMI 1640培养基培养人胃癌细胞株HGC-27,分别采用大蒜素(3μg·L^-1)联合白细胞介素6(IL-6,50 ng·mL^-1)、大蒜素(3μg·L^-1)、IL-6(50 ng·mL^-1)处理,另设空白对照组。采用实时荧光定量聚合酶链式反应（RT-qPCR）检测各组胃癌HGC-27细胞E-cadherin、vimentin及核因子-κB（NF-κB）P65的mRNA表达;Wst-3-2H-四唑单钠盐（CCK-8）实验、细胞划痕实验及细胞侵袭实验检测各组胃癌HGC-27细胞增殖、迁移和侵袭能力。结果 RT-qPCR显示,与空白对照组比较,大蒜素组E-cadherin mRNA表达上调,vimentin及NF-κB mRNA表达下调;IL-6组E-cadherin mRNA表达下调,vimentin及NF-κB P65的mRNA表达上调（均P<0.05）。与IL-6组比较,大蒜素联合IL-6组E-cadherin mRNA表达上调,vimentin与NF-κB mRNA表达下调（均P<0.05）。CCK-8实验结果显示,与空白对照组比较,大蒜素组胃癌HGC-27细胞被明显抑制,IL-6组具有明显增殖优势;与IL-6组比较,大蒜素联合IL-6组细胞被抑制（均P<0.05）;划痕实验中,与空白对照组比较,12,24 h大蒜素组胃癌细胞迁移能力明显减弱;与IL-6组比较,大蒜素联合IL-6组划痕愈合百分比缩小（均P<0.05）。细胞侵袭实验中,与空白对照组比较,大蒜素组侵袭数目减少,IL-6组侵袭数目明显增多;大蒜素联合IL-6组细胞侵袭能力较IL-6组明显减弱（均P<0.05）。结论大蒜素能明显抑制胃癌细胞EMT,减弱其增殖、迁移及侵袭能力,该过程可能与调节NF-κB信号通路有关。更多还原

【Abstract】 Objective To investigate the inhibitory effects and mechanism of allicin on the epithelial-mesenchymal transition（EMT） in gastric cancer cells. Methods Human gastric cancer line HGC-27 cell was routinely cultured in RPMI 1640 medium containing 10% fetal bovine serum.The HGC-27 cell was separated into allin(3 μg·L^-1) group,IL-6(50 ng·L^-1) group,or allin(3 μg·L^-1) combined with IL-6(50 ng·L^-1) group. In addition,the blank control group was set in parallel.By RT-qPCR technology,we detected the expression of E-cadherin,Vimentin and NF-κB P65.Wst-3-2 H-tetrazole menosodium salt（CCK-8） experiment,Wound Healing experiment,and cell invasion experiment were used to test the multiplication,removal and aggression ability of HGC-27 cells in each group. Results RT-qPCR showed that E-cadherin mRNA expression was up-regulated,and Vimentin and NF-κB mRNA expression was down-regulated in allicin group when compared with the blank control group. In IL-6 group, E-cadherin mRNA expression was down-regulated,and Vimentin and NF-κB P65 mRNA expression was up-regulated（all P<0.05）compared with blank control group. Compared to the IL-6 group,Allicin combined with IL-6 up-regulated mRNA expression of E-cadherin,and down-regulated the mRNA expression level of Vimentin and NF-κB mRNA（P<0.05）. CCK-8 experiment results showed that,the allicin group has a significant inhibitory effect on gastric cancer HGC-27 cells,and the IL-6 group has obvious proliferation advantages when compared with blank control group. Compared with IL-6 group,allicin combined with IL-6 group also has inhibitory effect.The scratch test showed that compared with the blank control group,the migration capacity of gastric cancer cells was significantly weakened after allicin treatment for 12 or 24 hours. Compared with the IL-6 group,the percentage of scratch healing in the allicin combined with IL-6 group was reduced（all P<0.05）. Cell invasion experiments showed that,compared with the blank control group,the number of invasions in the allicin group was decreased,the number of IL-6 invasion was increased significantly,and the invasion ability of allicin combined with IL-6 group was significantly weaker than that of IL-6 group（all P<0.05）. Conclusion Allicin can significantly inhibit EMT in the gastric cancer cells, and attenuate their proliferation,migration and invasion,and this process may be related to NF-κB signaling pathway.更多还原