【摘要】 目的建立高效液相色谱法同时测定五虫化瘀通络胶囊中阿魏酸、丹酚酸B和丹参酮ⅡA的含量。方法采用Agilent ZORBAX SB-C₁₈色谱柱（4.6 mm×250 mm,5μm）;以乙腈-0.1%磷酸作为流动相进行梯度洗脱;流速1.0 ml/min;检测波长:286 nm（检测阿魏酸和丹酚酸B）和270 nm（检测丹参酮ⅡA）;柱温为20℃。结果阿魏酸、丹酚酸B和丹参酮ⅡA分别在0.528～5.280μg/ml（R2=0.9999）、10.953～109.530μg/ml （R²=0.9996）、0.326～3.260μg/ml（R²=0.9994）浓度范围内与峰面积呈良好的线性关系;平均加样回收率分别为98.51%、99.51%、102.32%,RSD分别为1.29%、1.25%、1.41%。结论该方法稳定可靠,可用于同时测定五虫化瘀通络胶囊中3种成分含量。更多还原

【Abstract】 Objective To establish high performance liquid chromatography method for the simultaneous determination of ferulic acid,salvianolic acid B and tanshinone Ⅱ A in Wuchong Huayu Tongluo Capsules. Methods The analysis was performed on Agilent ZORBAX SB-₁₈ column（4.6 mm ×250 mm, 5 μm）; mobile phase was acetonitrile-0.1%phosphoric acid with gradient elution; flow rate was 1.0 ml/min; the detection wavelength were 286 nm（detected ferulic acid and salvianolic acid B） and 270 nm（detected tanshinone Ⅱ A）; column temperature was 20℃. Results Ferulic acid, salvianolic acid B and tanshinone ⅡA showed good linear relationships within the ranges of 0.528-5.280 μg/ml（R²= 0.9999）, 10.953-109.530 μg/ml（R²= 0.9996） and 0.326-3.260 μg/ml（R2= 0.9994）, whose average recovery rate were 98.51%, 99.51% and 102.32%, with the RSD of 1.29%, 1.25% and 1.41%, respectively. Conclusion The method is stable and reliable, and can be used for the simultaneous determination of three components in Wuchong Huayu Tongluo Capsules.更多还原