【摘要】 目的挖掘名老中医从瘀论治胃癌的组方规律,并基于网络药理学探讨核心药物的分子作用机制。方法计算机检索中国知网、万方、维普数据库2000年1月1日—2019年10月1日名老中医治疗胃癌的相关文献,结合人工检索书籍的方式收集名老中医处方;采用古今医案云平台(V1.5.6)对纳入处方的常用药对、核心药物组合等进行分析;采用中药系统药理学数据库和分析平台(TCMSP)、GeneCards及在线人类孟德尔遗传数据库(OMIM)筛选药物靶点和疾病靶点相互作用的有效靶点,并将有效靶点导入String数据库得到蛋白-蛋白相互作用(PPI)网络,利用R软件对有效作用靶点进行基因本体(GO)分析及京都基因和基因组百科全书(KEGG)通路富集分析。结果共纳入处方68首,得到8个核心药物,筛选出131个活性成分,获得62个核心药物有效作用靶点。GO功能分析集中在类固醇激素反应、对有毒物质的反应、细胞核受体活性等,KEGG分析可得主要作用通路为PI3KAkt信号通路、细胞凋亡、MAPK信号通路、缺氧诱导因子-1(HIF-1)信号通路等。结论本研究结果提示活血化瘀核心药物主要通过影响细胞周期、促进细胞凋亡、逆转化疗耐药性、影响血管生成对胃癌发挥作用,为后期实验研究提供了理论依据。更多还原

【Abstract】 Objective To explore the medication rules of famous old TCM doctors in treating gastric cancer from stasis,and to discuss the molecular mechanism of the core drugs based on network pharmacology. Methods Literatures related to the treatment of gastric cancer of famous old TCM doctors from January 1, 2000 to October 1, 2019 were screened in CNKI, Wanfang Data, VIP databases by computer, and the prescriptions of famous old TCM doctors were collected by manual search of books. Ancient and modern medical records cloud platform(V1.5.6) was used to analyze common medicine combinations and key prescriptions. Traditional Chinese medicine system pharmacology database and analysis platform(TCMSP), GeneCards and on-line Mendelian inheritance in man(OMIM) databases were used to screen the effective targets of drug target and disease target interaction, and the effective targets were imported in String database to get protein-protein interaction(PPI) network. The effective targets were analyzed by R software for gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) related pathway enrichment and analysis. Results Totally 68 prescriptions were included and eight core drugs were obtained, 131 active components and62 effective targets of core drugs were screened. The GO pathway enrichment analysis of the targets was mainly focused on steroid hormone response, reaction to toxic substances, nuclear receptor activity, etc. KEGG pathway enrichment analysis showed that the main action pathways were PI3 K-Akt signaling pathway,apoptosis, MAPK signaling pathway and hypoxia-inducible factor 1(HIF-1) signaling pathway, etc. Conclusion The results of this study suggest that the core drugs of promoting blood circulation for removing blood stasis mainly play a role in gastric cancer by influencing cell cycle, promoting apoptosis, reversing chemotherapy resistance and affecting angiogenesis, and they provide a theoretical basis for the later experimental study.更多还原