【摘要】 双光子显微镜是结合了激光扫描共聚焦显微镜和双光子激发技术的一种新精密仪器,具有低光毒性、高时空分辨率、高信噪比等优点,广泛应用于脑科学、免疫学、肿瘤、胚胎发育等生物医学相关研究领域。但双光子显微镜操作较复杂,对于新用户而言对设备的选用存在一定困惑。通过阐述双光子显微镜的工作原理、样品前期准备、成像难点及设备使用和日常维护要点,梳理双光子显微镜与其他同类成像类设备在成像原理、配置参数、成像特点及应用领域等方面的区别,总结双光子显微镜的应用特点及优势,为从事医学临床、教学及科研提供参考。更多还原

【Abstract】 Two-photon microscopy is one kind of new technique that combines confocal laser scanning microscopy and two-photon nonlinear excitation technique, which has the advantages such as low phototoxicity, high temporal-spatial resolution, high signal-to-noise ratio and so on. And it has been widely applied in the relevant research field includes brain science, immunology, tumor, embryonic development and other biomedicine. But the two-photon microscopy is more complex in the operations. Aiming at the confusion of new users on equipment selection, this paper briefly introduced the working principle of two-photon microscopy, sample preparation, imaging difficulties and the key points of equipment use and daily maintenance. And this paper also compared the differences of imaging principle, configuration parameters,imaging features and application field between two-photon microscopy and other similar imaging devices. Author summarized the application characteristics and advantages of two-photon microscopy so as to provide references for clinical practice, teaching and scientific research of medicine.更多还原