【摘要】 目的探究LINC00634在结直肠癌(CRC)中的表达及其与临床病理特征、免疫浸润以及预后的关系。方法从癌症基因组图谱(TCGA)数据库官方网站上下载CRC的RNA-seq数据及患者的临床病理信息,使用Mann-Whitney U检验分析CRC组织与正常组织之间的表达差异。分析LINC00634表达与患者临床病理特征的关系,利用Kaplan-Meier法分析LINC00634表达与患者预后的相关性。使用单因素和多因素Cox回归分析LINC00634表达与CRC患者临床预后关系。利用基因富集分析(GSEA)探索LINC00634在CRC中参与的信号途径。使用R 4.0.2软件评估LINC00634表达与肿瘤细胞免疫浸润丰度的相关性。结果 CRC样本中LINC00634表达水平显著高于正常组织,差异有统计学意义(P<0.05),LINC00634的表达与患者的年龄、临床分期和淋巴结转移分期显著相关(X~2=4.326、4.666、6.513,P<0.05)。LINC00634高表达组患者的总体生存率显著低于低表达组患者,差异有统计学意义(P<0.05)。多因素Cox回归分析显示,LINC00634可以作为CRC患者的独立预后因素(HR=5.377,95%CI:2.218～13.031,P<0.05)。GSEA富集分析结果提示,LINC00634主要参与DNA损伤修复、多条生化代谢通路,TGF信号通路,ECM受体相互作用通路等。LINC00634表达与CD8+T细胞、CD4+记忆T细胞呈正相关(R>0,P<0.05),而与嗜酸性粒细胞呈负相关(R<0,P<0.05)。结论 LINC00634在CRC中表达上调,参与多种癌症信号通路,可作为CRC患者的独立预后因素,是CRC新型分子标志物。更多还原

【Abstract】 Objective To investigate the expression of LINC00634 gene in colorectal cancer(CRC) and its relationship with clinicopathological characteristics,immune infiltration,and prognosis.Methods RNA-seq data of(CRC)and clinicopathological information of patients were downloaded from the official website of The Cancer Genome Atlas(TCGA)database,and the expression difference between CRC tissues and normal tissues were analyzed by Mann-Whitney U test. The association between the expression of LINC00634 and the clinicopathological characteristics. The correlation between the expression of LINC00634 and the prognosis of patients was analyzed by Kaplan-Meier method. The relationship between the expression ofLINC00634 and the clinical prognosis of patients with CRC was analyzed by univariate and multivariate Cox regression. Gene set enrichment analysis(GSEA)was used to explore the signaling pathway of LINC00634 in CRC. The association between LINC00634 expression and the abundance of tumor cell immune infiltration was evaluated using R 4.0.2 software. Results The expression level of LINC00634 in CRC samples was significantly higher than that in normal tissues,and the difference was statistically significant(P<0.05). The expression of LINC00634 was significantly correlated with the patient.s age,clinical stage and lymph node metastatic stage(X~2=4.326,4.666,6.513,P<0.05). The overall survival rate of patients with the high expression of LINC00634 was significantly lower than that of patients with the low expression of LINC00634,the difference was statistically significant(P<0.05). Multivariate Cox regression analysis showed that LINC00634 was an independent prognostic factor in patients with CRC(HR=5.377,95% CI:2.218～13.031,P<0.05). GSEA enrichment analysis results suggest that LINC00634 is involved in DNA damage repair,TGF signaling pathway,ECM receptor interaction pathway and other pathways. The expression of LINC00634 was positively correlated with CD8~+T cells,CD4~+ memory T cells(R>0,P<0.05),but negatively correlated with eosinophils(R<0,P<0.05). Conclusion LINC00634 is upregulated in CRC and participates in a variety of cancer signaling pathways. LINC00634 can be used as an independent prognostic factor for CRC patients and a new molecular marker for CRC.更多还原