【摘要】 目的观察细颗粒物(PM 2.5)对大鼠肺损伤的影响,探讨中药百令胶囊对肺损伤的保护作用。方法 2019年9—10月于河北省人民医院动物研究中心进行实验。将40只Wistar雄性大鼠按随机数字表法分为空白对照组、PM2.5染毒组及PM2.5+百令胶囊低、中、高剂量组5组。除空白对照组外,其余4组每3天将PM2.5混悬液(10 m g/kg)经大鼠气管内滴入进行PM2.5染毒,共10次。在PM2.5染毒基础上,百令胶囊组分别以低(0.25 g/kg)、中(0.5 g/kg)、高(1 g/kg) 3种不同剂量的百令胶囊悬液每日连续给予大鼠灌胃,共28 d;通过HE染色观察各组大鼠肺组织炎性病理变化,并应用ELISA法测定血清中白介素10(IL-10)、IL-22蛋白含量,利用实时荧光定量PCR法检测大鼠肺组织IL-10、IL-22 mRNA的表达。结果大鼠肺组织病理检查发现,PM2.5染毒组可见大量慢性炎性细胞浸润,百令胶囊干预各组较PM2.5染毒组炎性细胞浸润情况明显减轻。与空白对照组比较,PM2.5染毒组大鼠血清IL-10水平明显降低(t/P=27.758/0.000),血清IL-22水平明显升高(t/P=28.190/0.000)。与PM2.5染毒组比较,百令胶囊干预各组随剂量的增加,血清IL-10水平逐渐升高(F/P=224.867/0.000),而血清IL-22水平则逐渐下降(F/P=149.115/0.000)。Pearson相关分析表明:血清IL-10与IL-22水平呈负相关(r=0.960,P<0.05)。实时荧光定量PCR法检测法结果显示,与空白对照组比较,PM2.5染毒组肺组织IL-10 mRNA表达水平明显降低(t/P=61.342/0.000),肺组织IL-22 mRNA水平明显升高(t/P=55.298/0.000);与PM2.5染毒组比较,百令胶囊干预各组随剂量的增加,肺组织IL-10 mRNA水平逐渐升高(F/P=1 131.931/0.000),而IL-22 mRNA水平则逐渐下降(F/P=604.257/0.000)。结论 PM 2.5短期暴露可造成大鼠肺部炎性损害,百令胶囊可通过促进IL-10的分泌及表达,以及抑制IL-22的生成,对肺部起到保护作用。更多还原

【Abstract】 Objective To observe the effect of fine particulate matter(PM 2.5) on lung injury in rats, and explore the protective effect of traditional Chinese medicine Bailing capsule on lung injury. Methods Experiments were conducted at the Animal Research Center of Hebei Provincial People’s Hospital from September to October 2019. According to the random number table, 40 Wistar male rats were divided into 5 groups: blank control group, PM2.5 poisoning group, and PM2.5+ Bailing capsule low, medium and high dose groups. Except for the blank control group, the other 4 groups were instilled PM2.5 suspension(10 mg/kg) into the trachea of rats every 3 days for PM2.5 exposure, 10 times in total. On the basis of PM2.5 exposure, the Bailing capsule group was given low(0.25 g/kg), medium(0.5 g/kg), and high(1 g/kg) three different doses of Bailing capsule suspension daily. The rats were given intragastric gavage continuously for 28 days. The inflammatory pathological changes in the lung tissues of each group of rats were observed by HE staining, and the serum interleukin 10(IL-10) and IL-22 protein contents were measured by ELISA method, and IL-10 and IL-22 were detected by real-time fluorescent quantitative PCR method in rat lung tissue mRNA expression.Results Pathological examination of the lung tissue of the rats found that a large number of chronic inflammatory cell infiltrations were seen in the PM2.5-infected group. The Bailing capsule intervention in each group was significantly less inflammatory cell infiltration than the PM2.5-infected group. Compared with the blank control group, the level of serum IL-10 in the PM2.5-exposed group was significantly reduced(t/P=27.758/0.000), and the level of serum IL-22 was significantly increased(t/P=28.190/0.000). Compared with the PM2.5-exposed group, the serum IL-10 level of the Bailing capsule intervention group gradually increased with the increase of the dose(F/P=224.867/0.000), while the serum IL-22 level showed a gradual decline(F/P=224.867/0.000). P=149.115/0.000). Pearson correlation analysis showed that serum IL-10 and IL-22 levels were negatively correlated(r=0.960, P<0.05). The results of real-time fluorescent quantitative PCR detection method showed that compared with the blank control group, the IL-10 mRNA expression level in the lung tissue of the PM2.5-exposed group was significantly reduced(t/P=61.342/0.000), and the IL-22 mRNA level in the lung tissue was significantly increased.(t/P=55.298/0.000). Compared with the PM2.5-exposed group, the levels of IL-10 mRNA in lung tissues gradually increased(F/P=1 131.931/0.000) in each group of Bailing capsule intervention with increasing doses, while the level of IL-22 mRNA showed a gradual decrease.(F/P=604.257/0.000).Conclusion Short-term exposure to PM 2.5 can cause inflammatory damage to the lungs of rats. Bailing capsule can protect the lungs by promoting the secretion and expression of IL-10 and inhibiting the production of IL-22.更多还原