【摘要】 目的探讨5%醋酸对宫颈脱落细胞形态学以及HPV-DNA检测结果的影响。方法用5%醋酸处理Hela细胞,分别在0、2、5、10、15、20、25、30 min观察细胞形态变化规律。此外,取5%醋酸处理宫颈脱落细胞10 min后进行TCT制片,然后进行巴氏染色,观察细胞形态变化。另取5%醋酸处理宫颈脱落细胞10 min后,提取DNA,并进行HPV-DNA检测。结果 5%醋酸处理前、后,Hela细胞、宫颈脱落细胞的形态学未见异常,HPV-DNA检测结果也未见差异(P>0.05)。结论 5%醋酸对宫颈脱落细胞TCT和HPV-DNA检测结果没有影响。更多还原

【Abstract】 Objective To investigate the influence of 5% acetic acid on morphology of cervical exfoliated cells and HPV-DNA detection results. Methods Hela cells were treated with 5% acetic acid, and the morphological changes were observed at 0, 2, 5, 10, 15, 20, 25 and 30 min, respectively. In addition, cervical exfoliated cells were treated for 10 min by 5% acetic acid, then were used to making TCT flaking and given Pap staining to observe the morphological changes of cells. Furthermore, after the cervical exfoliated cells were treated with 5% acetic acid for 10 min, the DNA were extracted to perform HPV-DNA detection. Results Before and after 5% acetic acid treatment, no significant difference was found in the morphology of Hela cells and cervical exfoliated cells, and HPV-DNA detection results showed no difference(P>0.05).Conclusion The 5% acetic acid had no effect on the results of TCT and HPV-DNA detection in cervical exfoliated cells.更多还原