【摘要】 目的:应用Cirrus HD-OCT 5000对正常眼黄斑及视盘血流参数进行血管成像测量,评估其可重复性及再现性。方法:纳入40只正常眼进行前瞻性研究。操作者A于一周内3天的同一时段（T1,T2,T3）对正常受试者的同一眼黄斑及视盘进行3次血管成像扫描,扫描过程中均开启FastTrac^TM图像跟踪功能,操作者B在T2时间点再次对受试者同一眼进行相同程序扫描,使用Angio Plex Metrix^TM 量化软件（版本10.0）自动测量黄斑和视盘的血管长度密度（vessel length density,VD）和血管灌注密度（vascular perfusion density,PD）。应用单因素方差分析或非参数检验比较3次扫描的VD、PD是否有差异。采用组内相关系数（intraclass correlation coefficient,ICC）、变异系数（coefficient of variation,CV）评价操作者A的可重复性,采用一致性相关系数（consistent correlation coefficient,CCC）、重复性系数（repeatability coefficient,CR）、CV评价操作者间的再现性。结果:操作者A对黄斑及视盘各区域3次扫描的VD、PD之间差异无统计学意义（P>0.05）。操作者A对黄斑和视盘VD、PD 3次扫描的ICC值分别为0.260～0.517、0.362～0.898,黄斑及视盘各区域VD、PD的CV值均<8.1%。黄斑和视盘VD、PD的操作者间CCC值分别为0.3130～0.5665、0.5149～0.7801;黄斑VD和PD的CR值分别为3.2212～4.6399、0.0574～0.0832;视盘VD和PD的CR值分别为2.0675～4.0630、0.0447～0.0730。黄斑CV值均<9.0%,视盘CV值均<6.9%。结论:非同日的同一时段视盘浅层血流参数具有较好的重复性及再现性,黄斑浅层血流参数的重复性及再现性相对较差。更多还原

【Abstract】 Objective:To evaluate the repeatability and reproducibility of quantification of macular and peri-papillary superficial capillaries in normal eyes using Cirrus HD-OCT 5000 angiography.Methods:Forty normal eyes were included in this prospective study.Angiography 3 mm × 3 mm scan protocol centered on the disc or macula of the same eye was repeated 3 times by operator A in the same period on 3 days （T1,T2,T3） within 1 week,and the FastTrac^TM image tracking function was turned on during the scan.Operator B repeated the same scan independently at T2 after operator A.Angio Plex Metrix^TM quantification software （version 10.0） was used to automatically measure the vessel length density （VD） and vascular perfusion density （PD） of macular and peri-papillary superficial capillaries.One-way analysis of variance or nonparametric test was used to compare the VD and PD measurements of the 3 scans.The intraclass correlation coefficient （ICC） and coefficient of variation （CV） were calculated to evaluate the intra-operator repeatability.Consistent correlation coefficient （CCC）,repeatability coefficient （CR）,and CV were calculated to evaluate the inter-operator reproducibility.Results:There was no statistically significant difference in the measurements of VD and PD between the 3 scans of the macular and peri-papillary superficial capillaries by operator A （P>0.05）.The ICC values for the 3 scans of VD and PD of the macular and peri-papillary superficial capillaries by operator A were 0.260–0.517 and 0.362～0.898,respectively.The CV values of the VD and PD of the macular and peri-papillary superficial capillaries were all <8.1%.The inter-operator CCC values of the macular and peri-papillary superficial capillaries were 0.3130–0.5665 and 0.5149～0.7801,respectively;the CR values of the VD and PD of macula were 3.2212–4.6399 and 0.0574–0.0832,respectively;the CR values of the VD and PD of optic disc were 2.0675–4.0630 and 0.0447–0.0730,respectively.The CV values of macular were all <9.0%,and the CV values of optic disc were all <6.9%.Conclusion:The macular and peri-papillary superficial capillaries parameters in the same period on different days have a good repeatability and reproducibility,while the superficial macular blood flow parameters have relatively poor repeatability and reproducibility.更多还原