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五味子乙素对铝中毒大鼠神经细胞凋亡及学习记忆能力的影响
Effect of Sch B on neuronal apoptosis and the learning and memory ability of rats with aluminum intoxication
【摘要】 目的研究五味子乙素对慢性铝中毒大鼠神经细胞凋亡及学习记忆能力的影响。方法用AlCl3连续喂养大鼠建立慢性铝中毒大鼠模型,用五味子乙素对铝中毒大鼠进行灌胃治疗30d后,Morris水迷宫检测大鼠的学习记忆能力,记录空间探索实验潜伏期和平台穿越次数,原位细胞凋亡检测法(TUNEL)检测大鼠大脑皮层和海马神经细胞凋亡。结果五味子乙素高剂量组大鼠空间探索实验潜伏期比模型组的缩短,平台穿越次数比模型组的增多,差异有统计学意义(P <0.05)。铝中毒模型组大脑皮层和海马神经细胞凋亡率比空白对照组均明显增多(P <0.01);五味子乙素中、高剂量组大脑皮层和海马神经细胞凋亡率比模型组均明显减少(P <0.01)。结论五味子乙素提高铝中毒大鼠学习记忆能力与降低大脑皮层和海马神经细胞凋亡率有关。
【Abstract】 Objective To study the effect of Schisandrin B(Sch B)on neuronal apoptosis and the learning and memory ability of rats with chronic aluminum intoxication. Methods Rats were continuously fed with AlCl3 to establish the rat models of chronic aluminum intoxication.After 30 days of intragastric treatment with Sch B,the rats were detected by Morris water maze for their learning and memory ability.The incubation period of spacial navigation testing and the times of crossing the testing platform were recorded.Terminal deoxynucleotidy transferase-mediated dUTP-biotin nick-end labeling(TUNEL)was used to detect the neuronal apoptosis in the cerebral cortex and hippocampus of rats. Results The incubation period of spacial navigation testing in the high-dose Sch B group was shorter than that of the model group,while the number of times of platform crossing were more than that of the model group,indicating significant differences(P <0.05).The model group had higher apoptosis rate of neurons in the cerebral cortex and hippocampus of rats than the blank control group(P <0.01).The medium-dose Sch B group and the high-dose Sch B group both had significantly lower apoptosis rate of neurons in the cerebral cortex and hippocampus of rats than the model group(P <0.01). Conclusion The effect of Sch B on the learning and memory ability of rats with aluminum intoxication was related to the decrease in neuronal apoptosis rate of cerebral cortex and hippocampus.
【Key words】 Schisandrin B; chronic aluminum intoxication; neuronal apoptosis; cerebral cortex; hippocampus; learning and memory;
- 【文献出处】 右江民族医学院学报 ,Journal of Youjiang Medical University for Nationalities , 编辑部邮箱 ,2021年05期
- 【分类号】R285.5
- 【被引频次】1
- 【下载频次】215