【摘要】 目的探讨细胞周期蛋白依赖性激酶抑制因子2A(CDKN2A)基因表达与胰腺癌(PC)临床病理特征的关系。方法选择2016年1月至2018年6月朝阳市第二医院保存的88例PC组织标本和30例正常胰腺组织标本,采用定量反转录聚合酶链反应法检测PC组织和正常胰腺组织中CDKN2A mRNA表达,分析CDKN2A基因表达与PC临床病理特征及患者2 a总生存率和疾病无进展生存率的关系。结果 PC组织和正常胰腺组织中CDKN2A mRNA相对表达量分别为1.43±0.56、2.03±0.87,PC组织中CDKN2A mRNA相对表达量显著低于正常胰腺组织(P <0.05)。以正常胰腺组织中CDKN2A mRNA相对表达量均值2.03为界值,PC组织中CDKN2A基因高表达者36例,低表达者52例。CDKN2A基因高表达与低表达患者的性别、年龄、肿瘤部位比较差异无统计学意义(P> 0.05),CDKN2A基因高表达与低表达患者的肿瘤直径、组织分化程度和肿瘤临床分期比较差异有统计学意义(P <0.05)。CDKN2A mRNA高表达和低表达患者2 a总生存率分别为66.67%(24/36)、34.62%(18/52),2 a疾病无进展生存率分别为58.33%(21/36)、23.08%(12/52); CDKN2A mRNA低表达患者2 a总生存率和2 a疾病无进展生存率显著低于高表达患者(P <0.05)。结论 PC组织中CDKN2A基因表达下调,且CDKN2A基因表达与肿瘤大小、组织分化程度、肿瘤临床分期及患者生存状况有关。更多还原

【Abstract】 Objective To investigate the relationship between the expression of cyclin-dependent kinase inhibitor 2A (CDKN2A) gene and clinicopathological features of pancreatic cancer (PC). Methods Eighty-eight specimens of PC cancer and thirty normal pancreatic tissues in the Second Hospital of Chaoyang from January 2016 to June 2018 were collected. The expression of CDKN2A mRNA in PC tissues and normal pancreatic tissues was detected by quantitative reverse transcription polymerase chain reaction. The relationship between the expression of CDKN2A gene and clinicopathological features of PC,2-year overall survival rate and progression free survival rate of patients with PC was analyzed. Results The relative expression of CDKN2A mRNA in PC tissues and normal pancreatic tissues was 1. 43 ± 0. 56 and 2. 03 ± 0. 87,respectively. The relative expression of CDKN2A mRNA in PC tissues was significantly lower than that in normal pancreatic tissues (P < 0. 05). The average relative expression of CDKN2A mRNA in normal pancreatic tissue was 2. 03. Taking 2. 03 as the cut-off value,there were 36 cases with high expression of CDKN2A gene and 52 cases with low expression of CDKN2A gene in PC tissues. There was no significant difference in gender,age and tumor location between CDKN2A high expression and low expression patients (P > 0. 05). There were significant differences in tumor diameter,histological differentiation and clinical stage between CDKN2A high expression and low expression patients (P < 0. 05). The 2-year overall survival rate in patients with high expression and low expression of CDKN2A mRNA was 66. 67% (24/36) and 34. 62% (18/52),respectively; and the 2-year progression-free survival rate was 58. 33% (21/36) and 23. 08% (12/52),respectively. The 2-year overall survival rate and 2-year disease progression-free survival rate of patients with low expression of CDKN2A mRNA were significantly lower than those of patients with high expression of CDKN2A mRNA (P < 0. 05). Conclusion CDKN2A gene expression is down-regulated in PC tissues,and the expression of CDKN2A gene is related to the tumor size,tissue differentiation,clinical stage and survival status of patients.更多还原