【摘要】 为了更好地开发利用井栏边草总黄酮,利用微波辐射预处理材料,用硝酸铝作为显色剂来测定总黄酮质量分数,并通过响应面法优化汽化剂用量、微波功率和微波辐射时间3个提取工艺参数.通过CCK-8法检测乙酸乙酯提取物对人结肠腺癌细胞(RKO)、人回盲肠癌细胞(HCT8)、肺癌细胞(A549)和肝癌细胞(HepG2)4种癌细胞的毒性.结果表明,微波辐射预处理提取井栏边草总黄酮的最优条件为汽化剂用量5 mL,微波功率630 W,微波辐射时间90 s,此条件下总黄酮得率为37.916 mg/g.井栏边草乙酸乙酯提取物对4种癌细胞的抑制程度各有不同,对RKO,A549和HepG2的抑制率随着浓度的增加而上升,对HCT8抑制作用较弱,样品浓度对抑制率几乎没有影响.研究结果可以为井栏边草总黄酮的提取和活性研究提供参考.更多还原

【Abstract】 In order to better exploit and utilize the total flavonoids of Pteris multifida Poir, the content of total flavonoids was determined by using aluminium nitrate as chromogenic reagent based on the materials pretreated with microwaves. Then, the three extraction parameters including vaporizer dosage, microwave power and microwave radiation time, were optimized by response surface methodology(RSM). The cytotoxicity of total flavonoids(ethyl acetate extract) to four kinds of cancer cells including human colonic adenocarcinoma cells(RKO), human ileocecal cancer cells(HCT8) lung cancer(A549) and liver cancer(HepG2) was detected by CCK-8. The results show that the optimal conditions were as follows: the dosage of vaporizer was 5 mL, microwave power was 630 W, microwave radiation time was 90 s, Under this conditions, the yield of total flavonoids was 37.916 mg/g. The inhibitory effects of ethyl acetate extract on four kinds of cancer cells were different. The inhibitory rate of RKO, A549 and HepG2 increased with the increase of concentration, and the inhibitory effect on HCT8 was weak, the concentration of the sample had little effect on the inhibition rate. The results can be used as reference for the extraction and activity study of total flavonoids from Pteris multifida Poir.更多还原