【摘要】 【目的】本文以水稻(Oryza sativa L.)辽星1号为材料,对其进行碱蓬内生菌(EF0801)侵染及稻瘟病感染,意在探讨碱蓬内生菌(EF0801)能否提高水稻幼苗对稻瘟病的抗性。【方法】对水稻进行碱蓬内生菌(EF0801)侵染及稻瘟病感染,测定其形态指标、光合色素含量、防御酶活性及MDA含量以及叶绿素荧光参数,并对其进行分析。【结果】水稻感染稻瘟病菌后,Chlb含量显著降低,光系统Ⅱ(PSⅡ)放氧复合体受到抑制,电子传递不平衡,光合效率下降,光合性能指数降低;但内生菌侵染的水稻幼苗在感染稻瘟病菌后形态指标优于内生菌未侵染的、同时其叶片防御酶(POD、CAT)活性增强、MDA含量降低、Cars含量增加以及叶片PSⅡ放氧复合体稳定、电子传递正常、光合性能指数增加、光合效率提高。【结论】碱蓬内生菌(EF0801)侵染可以使水稻幼苗防御酶活性增强,降低MDA含量,能够缓解稻瘟病对PSⅡ的伤害作用,提高水稻对稻瘟病的抗性。更多还原

【Abstract】 【Objective】In this paper, the endophyte and rice blast infected rice(Oryza sativa L.) Liaoxing 1 seedlings were used as tested materials to explore whether Suaeda salsa endophyte(EF0801) could improve rice seedlings tolerance to rice blast.【Methods】Morphological indicators, photosynthetic pigment content, defensive enzyme activity, MDA content and chlorophyll fluorescence parameters of rice seedlings with endophyte(EF0801)and/or rice blast were determined.【Results】Rice-blast infection significantly decreased Chlb content, inhibited the photosystem II(PSII) oxygen-evolving complex, altered the electron transfer, decreased the photosynthetic efficiency and the photosynthetic performance index; After infection with rice-blast, the morphological indicators of seedlings with endophytic infection were better than those with endophytic uninfection, moreover endophytic infection increased the defensive enzyme activity, decreased MDA content, increased Cars content, as well as maintained PSⅡ oxygen-evolving complex stability and normal electron transfer proceeds, meanwhile improved photosynthetic performance index and photosynthetic efficiency.【Conclusion】Infection by endophytes(EF0801) increased the activity of defensive enzymes in rice seedlings, reduced the content of MDA,alleviated the damage of rice-blast to PSⅡand improved the resistance of rice to rice-blast.更多还原