【摘要】 【目的】基于前期对短小芽孢杆菌SCU11的转录组测序结果,对在转录组数据中不同阶段的表达量存在较明显差异的候选sRNA Bpsr145进行鉴定及探究。【方法】通过Northern blotting和独立转录验证Bpsr145的表达,使用不同的程序对其序列保守性、二级结构以及靶标基因进行分析,另外利用电转化方法构建一个Bpsr145敲除突变体。【结果】证实Bpsr145是一个真实存在并独立转录的sRNA,且在不同的培养基中显示不同的表达谱,但Bpsr145敲除菌株和野生型菌株在生长情况和蛋白质谱中不存在显著差异。【结论】本研究鉴定了一个短小芽孢杆菌的sRNA,丰富了芽孢杆菌非编码RNA数据库,为研究非编码RNA介导的转录后调控提供了新的材料。更多还原

【Abstract】 【Objective】Based on previous transcriptome analysis on Bacillus pumilus SCU11,the candidate sRNA Bpsr145 with obvious differences at different stages need to be identified and analyzed.【Method】The expression of Bpsr145 was confirmed by Northern blotting and independent transcription. Then we used different programs to analyze the sequence conservation,secondary structure and target genes of Bpsr145. Then a knockout mutant strain was constructed via electroporation method.【Result】We confirmed that Bspr145 was a sRNA in B.pumilus SCU11 showing different expression profiles under different culture conditions. The growth kinetics and protein profile between widetype and Bpsr145 depletion strain did not show significant difference.【Conclusion 】The study confirmed the real transcription of sRNA Bpsr145 in B. pumilus,which increases the inventory of regulatory sRNAs in Bacillus and implies the important regulatory role of sRNA in B. pumilus.更多还原