【摘要】 旨在检测PPP1R3C在爱拔益加肉鸡不同组织中的表达情况,探究外源胰岛素和能量限饲对鸡体胰岛素敏感组织中PPP1R3C表达的影响。试验一,取不同发育阶段的雌性肉鸡(E14、E19、D7和D21,n=10)组织样,通过qRT-PCR技术检测PPP1R3C在不同时期胸肌中的表达;试验二,腹腔注射胰岛素(INS)或PBS,取两组注射后不同时间点(0、15、120和240 min,n=5)的D24雄性肉鸡组织样,检测PPP1R3C在肉鸡不同组织中的表达,探究外源胰岛素处理对肉鸡胰岛素敏感组织中PPP1R3C表达的影响;试验三,取D18雌性肉鸡,一组饲喂常规日粮(n=20),另一组饲喂限制30%能量的日粮(n=20),饲喂至48天屠宰取样,探究30%能量限饲对肉鸡PPP1R3C表达的影响;试验四,D7雌性肉鸡随机分为3组,对照组、15%能量限饲组和15%蛋白限饲组(n=10),分别饲养至D21屠宰取样,探究能量限饲是否具有剂量依赖性。结果表明:1)PPP1R3C在胸肌组织中高表达,其次是心和腿肌组织(P<0.05)。2)PPP1R3C表达量呈现了明显随鸡发育而升高的趋势。3)INS注射显著下调了胸肌中PPP1R3C的表达,在注射后120 min时PPP1R3C的表达显著低于0和15 min(P<0.05);PBS注射后胸肌中PPP1R3C的表达没有显著差异,在注射后120和240 min时,INS组PPP1R3C表达显著低于PBS组(P<0.05)。INS注射也降低了肝中PPP1R3C的表达,在INS处理15 min时PPP1R3C的表达已显著低于0 min(P<0.05);PBS注射后肝中PPP1R3C表达处于动态平衡,在注射后120 min时,INS组PPP1R3C表达显著低于PBS组(P<0.05)。胰岛素注射后在腹脂中出现了与胸肌和肝相反的结果,在胰岛素注射后15 min时PPP1R3C的表达显著高于0、120、和240 min(P<0.05),而0、120、和240 min之间没有显著差异;PBS注射后腹脂中PPP1R3C表达没有显著变化,在注射后15 min时,INS组PPP1R3C表达量显著高于PBS组(P<0.05)。4)30%能量限饲导致PPP1R3C在胸肌和肝中的表达均显著下调(P<0.05)。5)15%能量限饲和15%蛋白限饲不能显著影响胸肌中PPP1R3C的表达。以上研究表明,PPP1R3C在胸肌中表达最高,并随着个体发育表达上升,且外源胰岛素对PPP1R3C的表达效应具有明显的组织特异性。30%能量限饲可产生类似于外源胰岛素的效应,且能量限饲存在一定的剂量依赖性,本研究结果为进一步揭示鸡PPP1R3C功能奠定了基础。更多还原

【Abstract】 The purpose of this study was to detect the expression of PPP1 R3 C in different tissues of AA broilers and to explore the effects of exogenous insulin and energy restriction on the expression of PPP1 R3 C in insulin sensitive tissues of chickens. In experiment 1, tissue samples of female broilers at different developmental stages(E14, E19, D7 and D21, n=10) were taken to detect the expression of PPP1 R3 C in chest muscle by qRT-PCR technique; In experiment 2, the tissue samples of D24 male broilers at different time points(0, 15, 120 and 240 min, n=5) after intraperitoneal injection of insulin or PBS were taken, to detect the expression of PPP1 R3 C in different tissues of broilers, and to explore the effect of exogenous insulin treatment on the expression of PPP1 R3 C in insulin sensitive tissues of broilers; In experiment 3, one group of D18 female broilers was fed with conventional diet(n=20) and the other group was fed diet with 30% energy restriction(n=20). After feeding for 48 days, slaughtering samples were taken to explore the effect of 30% energy restriction on the expression of PPP1 R3 C in broilers; In experiment 4, D7 female broilers were randomly divided into 3 groups: control group, 15% energy restriction group and 15% protein restriction group(n=10). Slaughtering samples were taken from D21 female broilers to explore whether energy restriction was dose-dependent. The results showed that: 1) PPP1 R3 C was highly expressed in chest muscle, followed by heart and leg muscle(P<0.05). 2) The expression of PPP1 R3 C showed a increasing trend with the growth and development of chicken. 3) Insulin injection significantly down-regulated the expression of PPP1 R3 C in chest muscle, and the expression of PPP1 R3 C at 120 min after insulin injection was significantly lower than that at 0 and 15 min(P<0.05); There was no significant change in the expression of PPP1 R3 C in chest muscle after PBS injection, the expression of PPP1 R3 C in INS group was significantly lower than that in PBS group at 120 and 240 min after injection(P<0.05). Insulin injection also decreased the expression of PPP1 R3 C in liver, and the expression of PPP1 R3 C after insulin treatment for 15 min was significantly lower than at 0 min(P<0.05); The expression of PPP1 R3 C in liver was in dynamic balance after PBS injection, at 120 min after injection, the expression of PPP1 R3 C in INS group was significantly lower than that in PBS group(P<0.05). The expression of PPP1 R3 C in abdominal fat was opposite to that in chest muscle and liver after insulin injection, the expression of PPP1 R3 C at 15 min after insulin injection was significantly higher than that at 0,120 and 240 min(P<0.05), but there was no significant difference among 0, 120, 240 min; There was no significant change in the expression of PPP1 R3 C in abdominal fat after PBS injection, the expression of PPP1 R3 C in INS group was significantly higher than that in PBS group at 15 min after injection(P<0.05). 4) The expression of PPP1 R3 C in chest muscle and liver was significantly down-regulated by 30% energy restriction(P<0.05). 5) 15% energy restriction and 15% protein restriction did not significantly affect the expression of PPP1 R3 C in chest muscle. The study results showed that the expression of PPP1 R3 C was the highest in the chest muscle, and increased with the development of broilers, and the effect of exogenous insulin on the expression of PPP1 R3 C had obvious tissue specificity. At the same time, the results of energy restriction showed that 30% energy restriction could produce an effect similar to that of exogenous insulin, and the energy restriction had a certain dose dependence, which laid a foundation for further revealing the function of chicken PPP1 R3 C.更多还原