【摘要】 本研究旨在通过甘薯辐射诱变技术获得甘薯有益突变体,丰富甘薯种质资源。本实验以甘薯主栽品种‘商薯19’为试材,用60Coγ射线对其胚性悬浮细胞进行辐照处理,辐照剂量为80 Gy,辐照6周后,将存活下来的细胞团转入含有1.0 mg/L ABA的MS固体培养基上诱导分化,再转入MS培养基获得完整植株138株。通过对辐照后代的顶叶色、叶脉色、顶叶形、叶形、顶端茸毛、薯形、烘干率、可溶性糖含量等性状调查和测定,发现1个株系(‘S19Y038’)顶叶色等性状发生变化,3个株系(‘S19Y116’‘、S19Y215’、‘S19Y219’)烘干率显著提高,分别比对照提高5.98%、5.65%和3.32%,1个株系(‘S19Y013’)可溶性糖含量显著提高,比对照提高17.81%。这些优异性状的突变体丰富了甘薯种质资源,在甘薯优质品种的选育中具有很好的应用前景。更多还原

【Abstract】 The purpose of the study is to obtain beneficial mutants of sweetpotato by radiation mutation technology, and enrich sweetpotato germplasm resources. In this experiment, the main sweetpotato variety‘Shangshu 19’was used as test material. The embryogenic suspension cells were irradiated with60 Co γ ray and the irradiation doses was 80 Gy. After 6 weeks of irradiation, the surviving cells were transferred into MS solid medium containing 1.0 mg/L ABA to induce differentiation, and then transferred into MS medium to obtain 138 complete plants. Through investigating and determining the top leaf color, vein color, top leaf shape, leaf shape,top villi, potato shape, drying rate and soluble sugar content of the irradiated progenies, it was found that the top leaf color of one line(‘S19 Y038’) changed, and the drying rate of three lines(‘S19 Y116’,‘S19 Y215’and‘S19 Y219’) were increased significantly by 5.98%, 5.65% and 3.32%, respectively. The soluble sugar content of one strain(‘S19 Y013’) was significantly increased by 17.81% compared with that of the control. These mutants with excellent characters could enrich the sweetpotato germplasm resources and have a good application prospect in breeding high-quality sweetpotato varieties.更多还原