【摘要】 目的探讨多囊卵巢综合征(PCOS)大鼠卵巢组织骨形态发生蛋白-15(BMP-15)及子宫内膜糖蛋白因子(Glycodelin-A(Gd-A)的表达,分析PCOS卵泡发育障碍和子宫内膜容受性下降的机制。方法选择21日龄SD雌性清洁级大鼠75只,随机分为对照组(25只)和PCOS组(50只,DHEA皮下注射构建),通过免疫组化染色观察BMP-15及卵泡刺激素受体(FSHR)在对照组和PCOS组卵巢组织的表达及不同受孕时间Gd-A和白血病抑制因子(LIF)在子宫内膜的定位表达,并测定宫腔冲洗液中Gd-A和LIF浓度。结果 PCOS组卵巢组织BMP-15和FSHR表达明显低于正常对照组,差异有统计学意义(P<0.01)。对照组大鼠子宫内膜Gd-A及LIF的阳性表达随受孕时间增加逐渐上升,而同时期PCOS组大鼠子宫内膜Gd-A及LIF的阳性表达随受孕时间增加改变幅度并不明显,但明显低于对照组,差异均有统计学意义(P<0.05)。宫腔冲洗液Gd-A和LIF浓度变化与子宫内膜表达结果类似。结论 PCOS大鼠卵巢BMP-15表达降低与PCOS卵泡发育不良有关,子宫内膜Gd-A合成异常是PCOS子宫内膜容受性降低原因之一。更多还原

【Abstract】 Objective To investigate the mechanism of follicular dysplasia and decreased endometrial receptivity of PCOS by studying the expression of BMP-15 in ovarian tissue and GdA in endometrium of PCOS model rats. Methods 21 days old SD rats were randomly divided into control group(25 rats) and PCOS model group(50 rats, were constructed with DHE). Immunohistochemical staining was used to observe the expression of BMP-15 and FSHR in the ovarian tissue of the control group and the PCOS model group, as well as the expression of GD-A and LIF in the endometrium at different conception time. Results The expression of BMP-15 and FSHR in ovarian tissue of model group was significantly lower than that of control group, and the difference was statistically significant(P<0.01). The positive expression of LIF and Gd-A in endometrium of normal rats gradually increased with prolongation of pregnant time, and the levels of GdA and LIF in the uterine flushing also has the same result. But at the same time the positive expression GdA and LIF in endometrium and the levels of Gd-A and LIF in the uterine flushing of model rats were statistically lower than that of normal rats at the same time, and no obviously changing with the increase of pregnant time. Conclusion Decreased expression of BMP-15 in ovary was related to the follicular dysplasia of PCOS, and the abnormal synthesis of Gd-A was one of reasons of decreased endometrial receptivity of PCOS.更多还原