【摘要】 针对生命科学、生物医药、纳米科技等领域日益增长的单粒子水平纳米颗粒表征需求,本课题组将瑞利散射和鞘流单分子荧光检测技术结合,成功研发了纳米流式检测技术(nano-flow cytometry,nFCM),实现单个纳米颗粒(7～500nm)以及细胞外囊泡、病毒、细菌、亚细胞器等天然生物纳米颗粒的粒径及其分布、颗粒浓度和生物化学性状的高灵敏、高选择性、高通量检测.本文对nFCM研发的重要性、挑战及如何实现超灵敏检测进行探讨,对基于该技术所发展的一系列生化分析新技术和方法进行综述,并对nFCM的应用前景进行展望.更多还原

【Abstract】 Developing rapid and sensitive techniques for quantitative and multi-parameter analysis of single nanoparticles is important for the advancements of life sciences,biomedicine,and nanotechnology.By integrating strategies for single-molecule fluorescence detection in a sheathed flow with approaches for efficient light scattering detection,Xiaomei Yan’s research group in Xiamen University has developed a nano-flow cytometry(nFCM).Synthetic nanoparticles(7-500 nm)and naturally occurring biological nanoparticles such as extracellular vesicles,viruses,bacteria and subcellular organelles can be analyzed at single-particle level in a high throughput manner,so that their size and size distribution,particle concentration and biological properties can be quickly measured.In this article,the significance,challenges and efforts toward sensitivity enhancement of nFCM are discussed.A series of bioanalytical techniques and methods developed upon nFCM are reviewed,and future improvement and applications of nFCM are envisioned.更多还原