【摘要】 背景:研究已证实部分生物医用镁合金产品具备抑菌性能,但是具体抑菌机制尚不明确。目的:探讨新型生物医用镁合金支架材料的体外抑菌性能及可能抑菌机制。方法:制取新型镁合金支架材料浸提液,以大肠杆菌及金黄色葡萄球菌为实验菌,通过浸提液接触培养的方式定量评估材料的相对抑菌率,通过扫描电镜观察细菌形态定性评估材料的抑菌性能,通过接触培养方式检测碱性磷酸酶、电导率、钾离子、细菌内外核酸及蛋白质含量等指标,初步探索新型镁合金支架材料可能的抑菌机制。结果与结论:①新型镁合金支架材料浸提液在与细菌接触培养的12 h内,对大肠杆菌的抑菌率在56.23%-79.72%之间,对金黄色葡萄球菌的抑菌率在62.34%-76.07%之间;②扫描电镜显示,与材料浸提液接触培养的两种细菌形态皱缩,体积变小,分布稀少;③材料浸提液对两种细菌细胞外环境中的碱性磷酸酶含量无影响,提高了两种细菌细胞外环境中的电导率、钾离子含量;④材料浸提液对大肠杆菌细胞外环境中的核酸与蛋白质含量无影响,提升了金黄色葡萄球菌细胞外环境中的核酸与蛋白质含量;⑤材料浸提液可抑制两种细菌内的核酸含量,对大肠杆菌细胞内可溶性蛋白含量无影响,抑制金黄色葡萄球菌细胞内可溶性蛋白合成;⑥结果表明,新型镁合金支架材料在体外具有一定的抑菌性能,对金黄色葡萄球菌的抑制作用强于大肠杆菌,其抑菌机制可能是破坏了细菌细胞膜的通透性,影响了细菌内核酸及蛋白质的合成。更多还原

【Abstract】 BACKGROUND: It has been confirmed that some biomedical magnesium alloy products have antibacterial properties, but the specific antibacterial mechanism is still unclear. OBJECTIVE: To investigate the antibacterial properties of biomedical Jiao Da Bio-Magnesium scaffold in vitro and explore possible mechanism.METHODS: Jiao Da Bio-Magnesium porous scaffold material extract was prepared. As the most common bacteria causing orthopedic implants infection, Escherichia coli and Staphylococcus aureus were selected for testing. The bacteriostasis rate was quantitatively evaluated by contact culture of the extraction solution. The bacteriostasis performance of the material was qualitatively evaluated by observing the bacterial morphology through scanning electron microscope. The alkaline phosphatase, conductivity, potassium ion, nucleic acid and protein content in bacterial extracellular liquid environment were detected. The possible antibacterial mechanism of Jiao Da Bio-Magnesium porous scaffold material extract was preliminarily explored. RESULTS AND CONCLUSION:(1) The bacteriostasis rate of Jiao Da Bio-Magnesium porous scaffold extract cultured with Escherichia coli for 12 hours ranged from 56.23% to 79.72%, while the Staphylococcus aureus group ranged from 62.34% to 76.07%.(2) Under scanning electron microscope, wizened form, smaller volume and scarcer distribution were observed.(3) The material extract had no effect on the content of alkaline phosphatase in the extracellular environment of the two bacteria, but increased the electrical conductivity and potassium ion content in the extracellular environment of the two bacteria.(4) The material extract had no effect on the content of nucleic acid and protein in the extracellular environment of Escherichia coli, and increased the content of nucleic acid and protein in the extracellular environment of Staphylococcus aureus.(5) The material extract could inhibit the nucleic acid content of the two bacteria, but had no effect on the soluble protein content of Escherichia coli cells, and inhibited the synthesis of soluble protein in Staphylococcus aureus cells.(6) Results suggested that Jiao Da Bio-Magnesium porous scaffold material has certain antibacterial properties in vitro, and the inhibitory effect on Staphylococcus aureus is stronger than that on Escherichia coli. The possible antibacterial mechanism is speculated that it can change the permeability of bacterial cell membrane and affect the synthesis of bacterial nucleic acids and proteins.更多还原