【摘要】 背景:癌症在世界范围内越来越普遍,影响了世界上1/3的人口,其发病率和死亡率都很高。随着对癌症分子生物学特点的不断认识,新的癌症治疗方案不断出现,其中单克隆抗体靶向药物治疗和免疫治疗给癌症患者带来了新希望。但是由于缺乏良好的模型,阻碍学者们更好地了解肿瘤的生物学特性和发现真正的药物靶点。有效的体外肿瘤模型对于抗癌药物生物筛选具有重要意义。目的:总结诱导多能干细胞在肿瘤疾病中的应用与前景。方法:检索PubMed、Web of Science和CNKI数据库等收录的有关诱导多能干细胞的文章,中文检索词为"诱导多能干细胞,肿瘤疾病,基因编辑,再生医学",英文检索词为"induced pluripotent stem cells,tumor diseases,gene editing,regenerative medicine",根据纳入与排除标准对所有文章进行初筛后,保留相关性较高的文章进行综述。结果与结论:诱导多能干细胞具有来源广、可直接从人外周血分离、在体外可诱导分化为肿瘤组织等优点,因此有越来越多将诱导多能干细胞用于疾病建模和药物筛选的尝试和探索。随着基因编辑技术(CRISPER/Cas9)的不断发展,将干细胞进行基因编辑并用于治疗肿瘤性疾病成为可能。更多还原

【Abstract】 BACKGROUND: Cancer is becoming more common worldwide, affecting one third of the world’s population, and its morbidity and mortality are high. With the deep understanding of the cancer molecular biology, new cancer treatment programs continue to appear, and monoclonal antibody targeted drug therapy and immunotherapy have brought new hope to cancer patients. However, the lack of good models has hindered our understanding of tumor biology and the discovery of real drug targets. Effective in vitro tumor models are of great significance for the biological screening of anticancer drugs.OBJECTIVE: To summarize the application and prospect of induced pluripotent stem cells in tumor diseases.METHODS: PubMed, Web of Science and CNKI databases were searched for the articles concerning the induced pluripotent stem cells. The keywords were "induced pluripotent stem cells, tumor diseases, gene editing, regenerative medicine" in English and Chinese, respectively. After screening based on the inclusion and exclusion criteria, the articles with high relevance were included for review RESULTS AND CONCLUSION: Induced pluripotent stem cells have the advantages of wide sources, direct isolation from human peripheral blood, and being induced to differentiate into tumor tissues in vitro. Thus, more and more induced pluripotent stem cells for disease modeling and drug screening are being explored. With the continuous development of gene editing technology(CRISPER/Cas9), gene editing of stem cells has become possible to treat tumor diseases.更多还原