【摘要】 目的探讨双黄连口服液对豚鼠宫内感染巨细胞病毒(GPCMV)孕鼠妊娠结局及感染致子代脑损伤的影响。方法将30只孕早期Hartley豚鼠随机分为正常组、GPCMV组和双黄连组,每组10只。正常组无特殊处理,GPCMV组和双黄连组均经腹腔接种GPCMV,双黄连组在接种的同时灌胃双黄连口服液(2 mL/kg),连续灌胃21 d。追踪观察孕鼠一般情况及仔鼠生长发育情况,存活仔鼠于5周后检测静脉血GPCMV-DNA载量、乙酰胆碱(Ach)、乙酰胆碱酯酶(AchE)活性及脑干听觉诱发电位(BAEP)。结果双黄连组孕鼠GPCMV感染率、早产孕鼠率及仔鼠GPCMV感染率均明显低于GPCMV组(P均<0.05),存活仔鼠数和仔鼠体质量明显高于GPCMV组(P均<0.05)。GPCMV组仔鼠静脉血GPCMV-DNA载量和AchE活性均明显高于正常组(P均<0.05),Ach水平明显低于正常组(P<0.05);双黄连组仔鼠静脉血GPCMV-DNA载量和AchE活性均明显低于GPCMV组(P均<0.05),Ach水平明显高于GPCMV组(P<0.05)。GPCMV组仔鼠Ⅲ波、Ⅴ波潜伏期和Ⅲ～Ⅴ波、Ⅰ～Ⅴ波峰间期均明显长于正常组(P均<0.05),Ⅲ波、Ⅴ波波幅均明显高于正常组(P均<0.05)。双黄连组仔鼠Ⅲ波、Ⅴ波潜伏期和Ⅲ～Ⅴ波、Ⅰ～Ⅴ波峰间期均明显短于GPCMV组(P均<0.05),Ⅲ波、Ⅴ波波幅均明显低于GPCMV组(P均<0.05)。结论双黄连口服液早期干预可抑制GPCMV引起的宫内感染,且可降低子代因GPCMV感染导致脑损伤的风险。更多还原

【Abstract】 Objective It is to investigate the effect effect of Shuanghuanglian oral liquid on the pregnancy outcome of guinea pigs infected with cytomegalovirus(GPCMV) in utero and brain damage of offspring caused by infection. Methods 30 early pregnant Hartley guinea pigs were randomly divided into three groups: normal group, GPCMV group and Shuanghuanglian group, each group had 10 guinea pigs. The normal group was given no special treatment. Both the GPCMV group and the Shuanghuanglian group were inoculated with GPCMV via the abdominal cavity. The Shuanghuanglian group was given the Shuanghuanglian oral liquid(2 mL/kg) by gavage at the same time as the inoculation, and the treatment was continuously lasted for 21 days. The general condition of pregnant mice and the growth and development of offspring was followed up and observed. After 5 weeks, the surviving offspring were tested for venous blood GPCMV-DNA load, acetylcholine(Ach), acetylcholinesterase(AChE) activity and brainstem auditory evoked potential(BAEP). Results The GPCMV infection rate, premature pregnancy rate and offspring GPCMV infection rate of pregnant mice in Shuanghuanglian group were significantly lower than those of GPCMV group(all P<0.05), and the number of surviving offspring and the weight of offspring were significantly higher than those of GPCMV group(all P<0.05). The GPCMV-DNA load and AChE activity in the venous blood of the offspring in the GPCMV group were significantly higher than those in the normal group(all P<0.05), and the Ach level was significantly lower than that in the normal group(P<0.05); the venous blood GPCMV-DNA load and AChE activity of the Shuanghuanglian group were significantly lower than those of the GPCMV group(all P<0.05), and the Ach level was significantly higher than that of the GPCMV group(P<0.05). The incubation period of wave Ⅲ and wave Ⅴ and the peak interval of wave Ⅲ～Ⅴ and wave Ⅰ～Ⅴ in GPCMV group were significantly longer than those in the normal group(all P<0.05), and the amplitude of wave Ⅲ and wave Ⅴ were significantly higher than those in the normal group(all P<0.05). The incubation period of wave Ⅲ, wave Ⅴ and the peak interval of wave Ⅲ～Ⅴ and wave Ⅰ～Ⅴ of Shuanghuanglian group were significantly shorter than those of GPCMV group(P<0.05), and the amplitude of wave Ⅲ and Ⅴ wave were significantly lower than that of GPCMV group(all P<0.05). Conclusion Early intervention with Shuanghuanglian oral liquid can inhibit the intrauterine infection caused by GPCMV and reduce the risk of brain injury caused by GPCMV infection in offspring.更多还原