【摘要】 随着化石燃料的广泛应用,多环芳烃污染问题日渐突显。为采用生物法处理多环芳烃污染、提高野生型菌种的降解能力,从北京某石化公司长期受石油污染的场地分离纯化出一株菲的高效降解菌,经鉴定为嗜麦芽寡养单胞菌,绘制了菌种进化树。利用氮离子诱变及常温等离子体复合诱变方法得到一株遗传稳定的高效降解菌株并对100 mg/L的菲进行降解处理,3 d后菲的降解率约为64%,5 d后基本完全降解。更多还原

【Abstract】 With wide application of fossil fuels,polycyclic aromatic hydrocarbon caused pollution has been becoming a serious problem.In order to improve the degradation ability of wild-type bacteria in biological treatment of polycyclic aromatic hydrocarbon,a strain of high-efficient phenanthrene degrading bacteria is isolated and purified from a long-term oil-contaminated site of a petrochemical company in Beijing.It is identified as maltophilic oligotrophomonas,and its strain evolutionary tree is drawn out.A genetically stable and highly efficient degrading strain is obtained by means of nitrogen ion mutagenesis and plasma mutagenesis at normal temperature,and is used to treat with 100 mg·L^-1 phenanthrene.It is found that phenanthrene has been degraded by about 64% in 3 days,and has been almost fully degraded after 5 days.更多还原