【摘要】 目的研究鄂药玛俐那的化学成分及其降脂活性。方法采用硅胶柱色谱、反相硅胶柱色谱、高效液相色谱及重结晶等技术对鄂药玛俐那的提取物进行纯化,利用化合物的理化性质和各种波谱技术分析鉴定其结构。结果从玛俐那果实中共分离得到11个化合物,化合物如下:gingerglycolipid A (1);2-oxo pomolic acid (2);cecropiacic acid (3);nikoenoside (4);garcimangosone D (5);ampelopsisionoside (6);saussureosides B (7);6-O-[(2E,6S)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]-α-D-glucopyranoside (8);6-O-[(2E,6S)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]-β-D-glucopyranoside (9);betulalbuside A (10);槲皮素(11)。细胞实验结果表明,化合物6能显著抑制HepG2细胞对三酰甘油的蓄积,无细胞毒活性;同时,化合物1、7对细胞内的三酰甘油也显示出了一定的抑制作用。结论 11个化合物均为首次从该植物中分离得到,该研究结果对进一步开发利用鄂药玛俐那奠定了基础。更多还原

【Abstract】 Objective To study the chemical components and the antihyperlipidemic activity of Crataegus dahurica Koehne ex C.K.Schneid.Methods The compounds were separated, purified and characterized by using various chromatographic methods including silica gel column chromatography(CC),reversed phase silica gel CC,HPLC and recrystallization with the spectroscopic and physicochemical properties analysis.Results 11 compounds were isolated from the fruits of C.dahurica.The compounds were as follows: gingerglycolipid A(1);2-oxo pomolic acid(2);cecropiacic acid(3);nikoenoside(4);garcimangosone D(5);ampelopsisionoside(6);saussureosides B(7);6-O-[(2E,6S)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]-α-D-glucopyranoside(8);6-O-[(2E,6S)-2,6-dimethyl-6-hydroxy-2,7-octadienoyl]-β-D-glucopyranoside(9);betulalbuside A(10);quercetin(11).The results of the inhibitory activity on triglyceride(TG) accumulation in HepG2 cells showed that compound 6 significantly inhibited the accumulation of TG in cells without inducing cytotoxic activity.In addition, compounds 1 and 7 also exhibited the moderately inhibitory activity on TG in cells.Conclusion 11 compounds were isolated from the fruits of C.dahurica for the first time.The results in this present study would be helpful for the further exploration and utilization of the edible herb.更多还原