【摘要】 为探明不同盐浓度胁迫对液体培养发菜生理生化指标的影响,本实验采用添加三个不同盐浓度(0、0.1、0.3 M)的BG-11培养基对发菜细胞进行培养,在培养0、5 d、10 d、15 d、25 d、30 d时,观察发菜的显微结构,同时测定发菜中叶绿素a、类胡萝卜素、藻蓝素、质膜透性、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)的活性.结果表明,0.1 M盐处理时有部分细胞仍可存活,且SOD活性相比未加盐的细胞显著升高;0.3 M盐处理对发菜细胞损伤最大,可显著降低叶绿素a和藻蓝素的含量,提高质膜透性和MDA含量,抑制发菜细胞的SOD活性,0.3 M盐添加的发菜细胞中类胡萝卜素在第5 d时含量高于其他组的细胞,之后随着时间增加急剧降低且低于其他组.本研究揭示发菜悬浮细胞对盐胁迫响应具有剂量效应,0.1 M盐添加时发菜细胞具有一定的耐盐性,0.3 M盐处理对发菜细胞造成不可逆的损伤.更多还原

【Abstract】 In order to explore the effects of salt stress on physiological and biochemical indexes of Nostoc flagelliforme in liquid culture, BG-11 mediums supplemented with three different salt concentrations(0,0.1,0.3 M) were used to culture Nostoc flagelliforme cells.The microstructure of Nostoc flagelliforme was observed at 0,5 days, 10 days, 15 days, 25 days and 30 days of culture.Meanwhile, chlorophyll a, carotenoid, phycocyanin, plasma membrane permeability and malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in Nostoc flagelliforme were measured.The results showed that some cells could survive in 0.1 M salt treatment, and the SOD activity was significantly increased compared with the cells without salt treatment; 0.3 M salt treatment had the greatest damage to Nostoc flagelliforme cells, which could significantly reduce the contents of chlorophyll-a and phycocyanin, improve the plasma membrane permeability and MDA content, and inhibit the SOD activity of Nostoc flagelliforme cells.The content of carotenoids in Nostoc flagelliforme cells added with 0.3 M salt was higher than that in other groups on the 5 th day, and then decreased sharply with the increase of time and was lower than that of other groups.This study revealed that the suspension cells of Nostoc flagelliforme had dose effect on response to salt stress.When 0.1 M salt was added, Nostoc flagelliforme cells had certain salt tolerance, and 0.3 M salt treatment caused irreversible damage to Nostoc flagelliforme cells.更多还原