【摘要】 目的比较两种商业化二抗在免疫组织化学染色中的一致性。方法以常见肿瘤组织样本为待检组织,选取18种常用免疫组织化学染色一抗,按国际专家特设委员会的建议设立阳性对照及阴性对照。使用DAKO自动免疫组织化学染色平台,其他实验条件一致,以两种不同二抗进行免疫组织化学染色,标准组二抗为DAKO聚合物系统（DAKO EnVision FLEX,High pH）,实验组二抗为Power-Stain^TM试剂盒(Power-Stain^TM 1.0 Poly HRP DAB Kit for Mouse+Rabbit)。染色后,采集图像,医师单盲法进行定位、定性以及半定量评价;两组选取同一区域,均以数字病理图像测量方法定量测量吸光度校正值、测量区域面积值以及灰度图阳性积分吸光度值,计算得到平均吸光度均值。结果两组所有样本染色的定位均准确,定性一致,两组半定量测量数据对两组强度比较、两组阳性百分率比较、两组平均吸光度均值比较,组间差异均无统计学意义。结论两种商业化二抗在免疫组织化学染色中具有良好的一致性。更多还原

【Abstract】 Objective To compare the consistency of immunohistochemical staining between the two commercial secondary antibodies. Methods Eighteen common immunohistochemical primary antibodies were selected and positive and negative controls were set up according to the recommendations from the AD Hoc Committee of International Experts. Under the same experimental conditions, the DAKO automatic immunohistochemical staining platform was used to test two different secondary antibodies for immunohistochemical staining. The standard group for the secondary antibody was provided by the DAKO polymer system（DAKO EnVision FLEX, High pH）, and the experimental group for the secondary antibody was provided by the Power-Stain^TM kit(Power-Stain^TM 1.0 Poly HRP DAB Kit for Mouse+Rabbit). Subsequently, the images were captured. A single-blind, positioning, qualitative and semi-quantitative scoring criterion was used for describing the positive stains by the experienced pathologist. Absorbance corrected values, measured area values and positive integral absorbance were detected by the digital pathology quantitative measurement in the same areas from the two groups. Then, the mean absorbance was calculated. Results The stains of all the samples from the two groups showed accurate location and consistent qualitative evaluation. No significant differences were found between the two groups in all the semi-quantitative scoring, including stain intensity, positive stain percentages and mean absorbance. Conclusion The two commercial secondary antibodies have strong consistency in the immunohistochemical staining.更多还原