【摘要】 基于甘薯(Ipomoea batatas (L.) Lam.)全基因组序列,利用生物信息学方法鉴定筛选了全基因组中的TCP(teosinte branched1/cincinnata/proliferating cell factor)转录因子,并分析了甘薯苗期在蔓割病菌胁迫及块根储藏期低温胁迫下TCP基因的表达差异。结果显示,甘薯基因组中共有27个TCP转录因子,其中ClassⅠ13个、ClassⅡ(CIN) 8个、ClassⅡ(CYC/TB1) 6个。甘薯TCP以单个基因或基因簇的形式不均匀分布在15条染色体上,其中6对基因存在潜在复制关系。甘薯ClassⅡ(CYC/TB1)转录因子含有R结构域,其氨基酸序列较为保守。在TCP家族中共发现10个保守基序(motif),基序的氨基酸序列长度为15～60个,其中motif 1含有TCP结构域,motif 3含有R结构域,TCP转录因子的保守基序种类在组间存在一定差异。逆境胁迫下甘薯的转录组数据分析结果表明,TCP转录因子在苗期受蔓割病侵染后有2个差异表达基因;在块根储藏期受低温胁迫下有11个差异表达基因。更多还原

【Abstract】 The unique TCP transcription factor family participates in various physiological and biochemical processes in plants. Based on the whole-genome sequence of Ipomoea batatas( L.) Lam.,we used bioinformatics to screen and identify TCP transcription factors and analyze differential expression of TCP genes in seedlings under Fusarium oxysporum f. sp.batatas stress and in tubers under low temperature stress during storage. Results showed that there were 27 TCP transcription factors in the I. batatas genome,including 13 in ClassⅠ,eight in Class Ⅱ( CIN),and six in Class Ⅱ( CYC/TB1). The I. batatas TCP genes were unevenly distributed in the 15 chromosomes and formed six pairs of genes with potential duplication relationships. The Class Ⅱ( CYC/TB1) I. batatas transcription factors contained an R domain with conserved amino acid sequences. There were 10 conserved motifs within the I.batatas TCP transcription factors,the amino acid lengths of which ranged from 15 to 60. Motif-1 contained a TCP domain and motif-3 contained an R domain. The types of conserved motifs for different groups varied to some extent. Analysis of transcriptomics data of I. batatas identified two differentially expressed genes under F. oxysporum f. sp. batatas infection during the seedling stage and 11 differentially expressed genes under low temperature stress during tuber storage. This study lays a foundation for the functional study of TCP genes and provides a reference for resistance breeding of I. batatas.更多还原