【摘要】 为制备及鉴定1-芘丁酸(1-pyrenebutyric acid)人工抗原。采用碳化二亚胺(EDC)法将1-芘丁酸与牛血清白蛋白(BSA)和鸡卵清白蛋白(OVA)进行偶联,合成人工免疫原PBA-BSA和人工检测抗原PBA-OVA;紫外扫描及SDS-聚丙烯凝胶电泳(SDS-PAGE)结果显示抗原制备成功;鼠源多抗血清的效价均已超过1 000,IC50=14.31 ng/mL;与多环芳烃1-芘甲醇、1-芘甲醛、芘的交叉反应率小于14.40%,与菲、萘、苯并芘、BSA以及OVA交叉反应率均小于0.05%。作者成功制备出了PBA-BSA抗原,并且得到了敏感性良好的鼠源多抗血清,为后期单克隆抗体的制备及免疫学快速检测方法的建立奠定基础。更多还原

【Abstract】 The preparation and identification of 1-pyrenebutyric acid artificial antigen was investigated in this paper.1-pyrenebutyric acid was coupled to bovine serum albumin(BSA) and chicken oval albumin(OVA) using the carbodiimide method(EDC) to synthesize the artificial immunogen PBA-BSA and artificial detection antigen PBA-OVA.The results of UV scanning and polypropylene gel electrophoresis(SDS-PAGE) showed that the antigen was successfully prepared.The potency of mouse-derived polyantisera was more than 1 000 with IC50 of 14.31 ng/mL. The cross-reactivity with polycyclic aromatic hydrocarbons 1-pyrene methanol, 1-pyrene formaldehyde and pyrene was less than 14.40%, and the cross-reactivity with phenanthrene, naphthalene,benzopyrene, BSA and OVA was less than 0.05%. PBA-BSA antigen was successfully prepared, and a sensitive murine multi antiserum was obtained, laying the foundation for the subsequent preparation of monoclonal antibodies and the establishment of rapid immunological detection methods.更多还原