【摘要】 对腓肠肌钝挫伤后大鼠恢复过程施加有氧运动进行恢复干预,通过分析肌电变化情况,探讨施加有氧运动对钝挫伤腓肠肌恢复的影响。60只雄性SD大鼠随机分为5组:正常对照组(A组)、自然恢复组(B组)、伤后48 h有氧运动组(C组)、伤后7 d恒定负荷有氧运动组(D组)、伤后7 d渐增负荷式有氧运动组(E组)。建模并于钝挫伤后2、5、7、10、14、21、28、30、35、56 d进行肌电图检测,观察大鼠钝挫伤腓肠肌恢复情况,记录静息电位、肌电异常自发活动出现的程度、复合肌肉动作电位(CMAP)的波幅和潜伏期的变化情况。结果显示:(1)自发电位结果:E组自发电位Daube积分分别在21、28、35 d显著低于C组,D组在28 d显著低于C组。(2)CMAP潜伏期比值:E组在14、28、35 d均显著低于B组,在14、21、28、35 d显著低于C组,在35 d显著低于D组。(3)CMAP波幅比值:D组在35、56 d显著高于B组,E组在10、14、28、35、56 d显著高于B组;D组和E组在10、14、21、28、35、56 d均显著高于C组;E组在10、56 d显著高于D组。结果表明:在腓肠肌钝挫伤恢复过程中,伤后48 h施加有氧运动,可加剧诱发大鼠自发电位异常,延长CMAP潜伏期,降低波幅恢复速度,不利于大鼠腓肠肌钝挫伤后的恢复及周围神经发生再支配;7 d后施加递增有氧运动和恒定负荷有氧运动可促进异常自发电位消失,缩短CMAP潜伏期,提高波幅恢复速度,有利于大鼠受损骨骼肌的修复及神经再支配。更多还原

【Abstract】 Aerobic exercise was applied to recovery intervention in the rats after blunt contusion of gastrocnemius muscle during the recovery period, so as to explore the effect of the recovery of gastrocnemius muscle with blunt contusion by conducting aerobic exercise via analyses of electromyographic changes. 60 male SD rats were randomly divided into five groups: normal control group(group A), natural recovery group(group B), aerobic exercise group 48 h after injury(Group C), constant load aerobic exercise group 7 d after injury(Group D), gradually increasing load aerobic exercise group 7 d after injury(Group E). The rats were sacrificed at 2 d, 5 d, 7 d, 10 d, 14 d, 21 d, 28 d, 30 d, 35 d and 56 d after blunt contusion, and were tested by electromyography. The recovery of gastrocnemius muscle was observed, the resting potential was recorded and the changes of CMAP were inducted during recovery. Results show that:(1) spontaneous potential: the Daube score of spontaneous potential in Group E was significantly lower than that in group C at 21 d, 28 d and 35 d, and that in group D was significantly lower than that in group C on 28 d.(2) CMAP latency ratio: the latency of group E was significantly lower than that of group B at 14 d, 28 d and 35 d, significantly lower than that of group C at 14 d, 21 d, 28 d and 35 d, and significantly lower than that of group D at 35 d.(3) CMAP amplitude ratio: the amplitude of group D was significantly higher than that of group B at 35 d and 56 d, and that of group E was significantly higher than that of group B at 10 d, 14 d, 28 d, 35 d and 56 d; the amplitude of group D and E was significantly higher than that of group C at 10 d, 14 d, 21 d, 28 d, 35 d and 56 d; the amplitude of group E was significantly higher than that of group D at 10 d and 56 d. The study indicates that: during the recovery process of gastrocnemius contusion, aerobic exercise 48 hours after injury can aggravate the abnormal spontaneous potential, prolong the latent period of CMAP, and reduce the recovery speed of amplitude, which is not conducive to the recovery of gastrocnemius contusion and reinnervation of peripheral nerve; after 7 days, incremental aerobic exercise and constant load aerobic exercise can promote the disappearance of abnormal spontaneous potential, shorten the latent period of CMAP, improve the recovery speed of amplitude, which is conducive to the repair and reinnervation of damaged skeletal muscle.更多还原