【摘要】 目的 研究血管内皮细胞生长因子反义寡核苷酸(VEGF-ASODN)和生存素(Survivin)反义寡核苷酸(Survivin-ASODN)联合转染对裸鼠体内Tca8113舌癌细胞的作用。方法 25只雌性裸鼠分为5组,每组5只,建立移植瘤模型,并注入相应的试剂。①联合ASODN组:每次在裸鼠瘤体及瘤周注入Survivin-ASODN、VEGF-ASODN各66μg,注射液体积为200μl,内含LipofectamineTM 2000和无血清1640液。②VEGF-ASODN组:同上述方法注射VEGF-ASODN 66μg。③Survivin-ASODN组:同法注射Survivin-ASODN 66μg。④脂质体组:同法注射等量脂质体200μl。⑤生理盐水(NS)组:注射等量NS200μl。3天注射1次,共7次。实验结束2天后,全麻下处死裸鼠,称取肿瘤重量并测量大小,肿瘤制作石蜡切片,免疫组织化学方法测VEGF、Survivin、PCNA、CD34表达水平,计算肿瘤微血管密度(microvessel density,MVD)和肿瘤增殖细胞核抗原标记指数(PCNA Label index,PLI)。结果 联合ASODN组裸鼠移植瘤的重量、体积、VEGF和Survivin蛋白、PCNA、CD34表达水平明显降低。结论 VEGF-ASODN和Survivin-ASODN联合转染Tca8113舌癌细胞,明显抑制VEGF和Survivin表达水平,并且抑制癌细胞生长和增殖。更多还原

【Abstract】 Objective:To study the effect of VEGF ASODN and Survivin ASODN co transfection on Tca8113 tongue cancer cells in nude mice.Methods:25 female nude mice were divided into 5 groups with 5 mice in each group.①In the combined ASODN group,66 μg survivin ASODN and 66 μg VEGF ASODN were injected into the tumor body and peritumoral area of nude mice.The volume of injection was 200 μl.It contains lipofectaminetm 2000 and serum-free 1640 solution.①VEGF ASODN group:VEGF ASODN 66 μg was injected in the same way.③Survivin ASODN group:survivin ASODN 66 μg was injected in the same way.④Liposome group:the same amount of liposome 200 μl was injected in the same way.⑤NS group:the same amount of ns 200 μl was injected.Once every 3 days,7 times in total.Two days after the end of the experiment,the nude mice were killed under general anesthesia.The weight of the tumor was measured and the size of the tumor was measured.The tumor was made into paraffin sections.The expression levels of VEGF,survivin,PCNA and CD34 were measured by immunohistochemistry.The microvessel density(MVD) and PCNA label index(PLI) of the tumor were calculated.Results:The weight,volume,VEGF and survivin protein,PCNA and CD34 expression of xenografts in nude mice were significantly decreased in the combined ASODN group.Conclusion:Co transfection of VEGF ASODN and Survivin ASODN can significantly inhibit the expression of VEGF and Survivin,and inhibit the growth and proliferation of Tca8113 tongue cancer cells.更多还原