【摘要】 目的：比较荧光定量聚合酶链式反应(PCR)检测结核杆菌脱氧核糖核酸(DNA)与涂片抗酸染色诊断肺结核的效果。方法：收集2017年1月至2020年12月在新乡市第一人民医院就诊的疑似肺结核患者95例的痰液标本127份，对所有痰液标本均实施荧光定量PCR检测结核杆菌DNA及涂片抗酸染色检测，以痰培养结果为金标准，比较两种检测方法的诊断效能。结果：痰培养结果显示，95例疑似肺结核患者中，61例患者确诊，确诊率为64.21 %。荧光定量PCR检测的准确度、特异度、灵敏度及阳性预测值、阴性预测值均明显高于涂片抗酸染色检测，差异均具有统计学意义(P<0.05)。结论：荧光定量PCR检测结核杆菌DNA对肺结核的诊断效能明显高于涂片抗酸染色检测。更多还原

【Abstract】 Objective To compare the effects of fluorescent quantitative polymerase chain reaction (PCR) detection of Mycobacterium tuberculosis deoxyribonucleic acid (DNA) and acid-fast staining of smears in the diagnosis of tuberculosis.Methods 127 sputum samples from 95 suspected tuberculosis patients who were treated at the First People’s Hospital of Xinxiang City from January 2017 to December 2020 were collected.All sputum samples were subjected to fluorescence quantitative PCR to detect Mycobacterium tuberculosis DNA and smears acid-fast staining test.The sputum culture result was used as the gold standard to compare the diagnostic efficacy of the two test methods.Results The results of sputum culture showed that among 95 suspected pulmonary tuberculosis patients,61 patients were diagnosed with a diagnosis rate of 64.21%.The accuracy,specificity,sensitivity,positive predictive value,and negative predictive value of fluorescent quantitative PCR detection were significantly higher than those of smear acid-fast staining detection,and the differences were statistically significant (P < 0.05).Conclusion The diagnostic efficiency of fluorescent quantitative PCR for detecting Mycobacterium tuberculosis DNA is significantly higher than that of acid-fast staining of smears,and its clinical application value is higher.更多还原