【摘要】 目的检测长链非编码RNA肿瘤易感候选者9(lncRNA CASC9)、微小RNA-758-3p(miR-758-3p)在肝细胞肝癌(hepatocellular carcinoma,HCC)血清中的表达及诊断价值。方法选取2018年6月至2020年2月于镇江市第三人民医院收治的HCC患者72例作为HCC组,另选取健康体检人员30例作为健康对照组。star Base v2.0在线软件预测lncRNA CASC9与miR-758-3p的结合位点;检测受试者血清中lncRNACASC9、miR-758-3p的表达,分析两者与HCC患者临床病理参数的关系,分析两者和甲胎蛋白(AFP)对HCC的诊断价值。结果 Star Base v2.0软件结果显示,lncRNA CASC9与miR-758-3p存在潜在的互补结合位点。与健康对照组相比,lncRNA CASC9在HCC患者血清中的表达升高(P <0.05),miR-758-3p在HCC患者血清中的表达降低(P<0.05)。HCC患者血清中lncRNA CASC9、miR-758-3p的表达与肝硬化、淋巴结转移和TNM分期有关(P<0.05)。ROC分析显示:lncRNA CASC9、miR-758-3p和AFP对HCC均有一定的诊断价值。三者联合应用的诊断价值较高,ROC-AUC为0.848(95%CI:0.767～0.937)。结论血清lncRNACASC9和miR-758-3p有可能成为HCC早期诊断的血清学标志物。更多还原

【Abstract】 Objective To detect the expression levels and diagnostic value of long non-coding RNA cancer susceptibility candidate 9(lncRNA CASC9)and micro RNA-758-3p(miR-758-3p)in serum of hepatocellular carcinoma(HCC).Method 72 patients with HCC who were treated in the third people′s hospital of Zhenjiang City from June 2018 to February 2020 were selected,and they were selected as HCC group.30 cases for medical examination of health personnel were selected as healthy control group.The binding sites of lncRNA CASC9 and miR-758-3p were predicted by Star Base v2.0 online software.The expressions of lncRNA CASC9 and miR-758-3p in the serum of the subjects were detected,and the relationship between them and clinic pathological parameters of HCC patients was analyzed.The diagnostic value of them and AFP on HCC were analyzed.Results The results of Starbase v2.0 software showed that there was a potential complementary binding site between lncRNA CASC9 and miR-758-3p.Compared with the healthy control group,the expression of lncRNA CASC9 in serum of HCC patients was increased(P<0.05),and the expression of miR-758-3p in serum of HCC was decreased(P<0.05).The expression of lncRNA CASC9 and miR-758-3p in serum of HCC patients were correlated with liver cirrhosis,lymph node metastasis and TNM stage(P<0.05).ROC analysis showed that lncRNA CASC9,miR-758-3p and AFP had certain diagnostic value for HCC.The combined application of the three methods had higher diagnostic value,and the ROC-AUC was 0.848(95%CI:0.767～0.937).Conclusion Serum lncRNA CASC9 and miR-758-3p may be used as serological markers for early diagnosis of HCC.更多还原