【摘要】 目的检测梅毒血清固定患者的NLRP3炎症小体及相关细胞因子的表达水平,探讨它们在梅毒血清固定形成过程中的作用。方法采用病例对照研究的方法,运用qRT-PCR检测梅毒血清固定患者及健康人的PBMCs中NLRP3炎症小体及相关细胞因子mRNA的表达,并用ELISA检测上述两组血清中细胞因子IL-1β和IL-18水平。结果梅毒血清固定患者PBMCs中NLRP3、ASC、Caspase-1、IL-1β和IL-18mRNA表达明显低于健康者,差异有统计学意义(均P <0.05),同时血清中IL-1β和IL-18的含量也低于健康者,差异有统计学意义(均P <0.05)。结论梅毒血清固定患者的NLRP3炎症小体及下游细胞因子表达下调,提示梅毒血清固定的形成可能与患者的天然免疫异常有关。更多还原

【Abstract】 Objective To detect the expression level of the NLRP3 inflammasome and the associated cytokines of syphilis serofast state,and to investigate the effect of NLRP3 inflammasome in the formation of syphilis serofast.Methods In a case-control study of 50 syphilis serofast patients and 50 healthy subjects,qRT-PCR was used to detect the expression of NLRP3 inflammasome and associated cytokines mRNA in peripheral blood mononuclear cells(PBMCs) of patients with syphilis serofast state and healthy subjects,and to detect the levels of cytokines IL-1β and IL-18 in the serum by ELISA.Results The expressions of NLRP3,ASC,Caspase-1,IL-1β and IL-18 mRNA in PBMCs of patients with syphilis serofast state were significantly lower than those of healthy subjects(all P<0.05),and the contents of IL-1β and IL-18 in serum of syphilis serofast state patients were also lower than those of healthy controls(both P<0.05).Conclusion The expression of NLRP3 inflammasome and downstream cytokines decreases in patients of syphilis serofast,and suggests that it maybe related to the natural immunity abnormality of these patients.更多还原