【摘要】 目的探讨5-HT₇受体在三叉神经节电刺激引起的硬脑膜血浆蛋白外渗（plasma protein extravasation,PPE）中的作用。方法建立三叉神经节电刺激偏头痛大鼠模型,35只大鼠随机分为7组:舒马曲坦(5-HT_1B/1D受体激动剂)阳性对照组、AS19（5-HT₇受体激动剂）5 mg/kg及10 mg/kg组、SB269970（5-HT₇受体拮抗剂）5 mg/kg及10 mg/kg组、二甲基亚砜及生理盐水阴性对照组。电刺激前30 min给药,刺激前5 min注射2%伊文思蓝用于标记硬脑膜PPE,用荧光显微镜拍摄并用灰度分析软件对PPE做定量分析,刺激侧与对侧荧光灰度值之比值为PPE率。结果舒马曲坦组PPE率较其他组明显降低（P <0.005）;AS19、SB269970组PPE率与阴性对照组相比差异均无统计学意义（P> 0.05）。结论 5-HT₇受体可能未参与三叉神经血管系统激活引起的硬脑膜PPE。更多还原

【Abstract】 Objective To investigate the role of 5-HT₇ receptors in dural plasma protein extravasation（PPE） induced by electrical stimulation of the trigeminal ganglion.Methods To construct migrainous rat model by means of electrical stimulation of the trigeminal ganglion.A total of 35 rats were randomly divided into 7 groups,sumatriptan（5-HT1B/1D receptor agonist） positive control group,AS19（5-HT₇ receptor agonist） 5 and 10 mg/kg groups,SB269970（5-HT₇ receptor antagonist） 5 and 10 mg/kg groups,dimethyl sulfoxide and normal saline negative control groups.All drugs were administered 30 minutes before electrical stimulation.2% Evans blue dye was injected intravenously as a marker of PPE 5 minutes before electrical stimulation.The assessment of PPE was measured using a fluorescence microscope equipped with a grating monochromator and a spectrophotometer.The ratio of the fluorescence intensity from the stimulated compared to the contralateral side was expressed as the PPE ratio.Results The PPE ratio of sumatriptan group was significantly lower than other groups（P <0.005）.No significant differencewas discovered among AS19,SB269970 and negative control groups（P> 0.05）.Conclusions 5-HT₇ receptors might not be involved in dural PPE induced by activation of the trigeminovascular system.更多还原