【摘要】 目的:研究核受体辅激活蛋白7(NCOA7)在缺氧条件下对口腔鳞癌细胞糖酵解过程的调节作用。方法:采用免疫荧光双染的方法检测口腔鳞癌组织中NCOA7和芳香烃受体(AhR)的表达。用氯化钴构建人舌鳞癌细胞株(SCC9)的缺氧模型。通过siRNA转染、RT-PCR和蛋白印迹等方法分析NCOA7与AhR的相互调节作用及对细胞糖酵解水平的影响。结果:NCOA7和AhR在口腔鳞癌组织细胞核中表达均高于癌旁组织(P<0.000 1)。在缺氧条件下,NCOA7的沉默增强了SCC9细胞的糖酵解水平(P<0.05),而激活AhR可以部分逆转这一过程(P<0.05)。结论:NCOA7在缺氧条件下可通过AhR干预口腔鳞癌的糖酵解水平。更多还原

【Abstract】 Objective: To investigate the regulation of nuclear receptor coactivator 7(NCOA7)on the glycolysis of oral squamous cell carcinoma cells under hypoxia. Methods: Immunofluorescence double staining was used to detect the expression of NCOA7 and Aryl hydrocarbon receptor(AhR) in OSCC and paracancerous tissues. A hypoxia model of human tongue squamous cell carcinoma cell line(SCC9) was established with cobalt chloride. siRNA transfection, RT-PCR and Western blotting were used to analyze the correlation between NCOA7 and AhR and its effect on glycolysis. Results: NCOA7 and AhR expression was significantly higher in OSCC tissue than that in paracancerous tissue(P<0.000 1). In anoxic environment, knocking down of NCOA7 aggravated the glycolysis level of SCC9 cells and activation of AhR could partly reverse this process(P<0.05). Conclusion: NCOA7 can intervene the glycolysis level of OSCC cells by targeting AhR under hypoxia.更多还原