【摘要】 目的:对比胃癌与癌前病变组织中K-ras蛋白表达的差异,分析K-ras诱导的细胞衰老与胃癌及癌前病变的关系。方法:收集138例管状腺癌患者的病理样本,进行HE染色和免疫组化染色,检测胃癌及癌旁石蜡包埋组织中K-ras、SA-β-Gal、P53、P16^INK4a蛋白的表达。结果:K-ras蛋白在胃癌组织中阳性表达率显著高于癌旁组织（95.7%与3.6%）（P<0.05）。胃癌组织及癌旁组织中K-ras阳性组SA-β-Gal、P53、P16^INK4a表达率均高于K-ras阴性组（均P<0.05）。结论:K-ras诱导的细胞衰老与胃癌的发生、发展密切相关。更多还原

【Abstract】 Objective:To compare the expression of K-ras protein in gastric cancer and precancerous lesions, and to analyze the relationship between K-ras-induced cellular senescence and gastric cancer and precancerous lesions.Methods:138 gastric tubular adenocarcinoma specimens were collected, and HE staining and immunohistochemical staining were performed.The expression of K-ras, SA-β-Gal, P53 and P16^{INK4 a} in gastric cancer and adjacent tissues were detected.Results:The positive expression rate of K-ras protein in gastric cancer tissues was significantly higher than that in adjacent tissues（95.7% vs.3.6%）（P<0.05）.The positive rates of SA-β-Gal, P53 and P16^{INK4 a} expression of K-ras positive group in gastric cancer tissues and adjacent tissues were higher than those in K-ras negative group（all P<0.05）.Conclusion:K-ras-induced cellular senescence is closely related to the occurrence and development of gastric cancer.更多还原