【摘要】 目的探讨沉默信息调节因子(SIRT1)基因对小鼠椎体骨质疏松的影响。方法将小鼠分为两组:软骨SIRT1基因未敲除小鼠组(A组,n=7);软骨SIRT1基因特异性敲除小鼠组(B组,n=7)。利用荧光定量聚合酶链反应及SIRT1免疫组化检测SIRT1基因的表达情况:通过对两组小鼠尾椎行Micro-CT扫描量化骨量体积百分比、骨小梁数量和骨小梁的厚度的改变;再采用HE染色、Masson染色评价椎体骨质结构的改变。结果 B组小鼠SIRT1 mRNA表达量为(2.61±1.11),明显低于A组(8.08±1.64)(P<0.01);B组SIRT1蛋白的免疫组化染色积分为(3.10±2.69),明显低于A组(5.07±2.99)(P<0.01)。 Micro-CT结果显示,A组小鼠骨量体积百分比为(37.96±8.54) %,显著高于B组的(28.39±6.27) %,差异有统计学意义(P<0.05);A组小鼠骨小梁数目(5.76±0.63)显著高于B组(5.15±0.50),差异有统计学意义(P<0.05);两组小鼠的骨小梁厚度差异无统计学意义(P>0.05)。HE染色和Masson染色结果显示,A组椎体骨质较B组明显密集。结论 SIRT1基因敲除减少了小鼠椎体骨量体积百分比、骨小梁数量,因此SIRT1基因可能对小鼠椎体骨量调节具有积极的作用。更多还原

【Abstract】 Objective To investigate the effect of SIRT1 knock-out on vertebral osteoporosis in mice. Methods The mice were divided into two groups: Wild type control group(group A, n=7); Cartilage SIRT1 knockout group(group B,n=7). FQ-PCR and SIRT1 immunohistochemistry were used to detect the expression of SIRT1 gene; Micro-CT scan was performed on the tail vertebrae of the two groups to quantify percentage of bone mass, number of trabecular bone and thickness of trabecular bone. HE staining and Masson staining were used to evaluate the changes of vertebral bone structure.Results The expression of SIRT1 m RNA in group B was(2.61±1.11), which was significantly lower than that of group A(8.08±1.64)(P<0.01). The immunohistochemical staining score of SIRT1 protein in group B was(3.10±2.69), which was significantly lower than that of group A(5.07±2.99)(P<0.01). Micro-CT results showed that the bone volume percentage of mice in group A [(37.96±8.54) %] was significantly higher than that in group B [(28.39±6.27) %], which was statistically significant(P<0.05); the number of trabecular bones in group A(5.76±0.63) was significantly higher than that of group B(5.15±0.50), which was statistically significant(P<0.05). There was no significant difference in trabecular thickness between two groups(P>0.05). The results of HE staining and Masson staining showed that the vertebral bone in group A was significantly denser than that in group B. Conclusion SIRT1 knockout reduced the volume percentage of vertebral body and the number of trabeculae in mice, so SIRT1 gene may have a positive regulatory effect on the bone mass of vertebral body in mice.更多还原