【摘要】 本实验采用Garre模型模拟单核细胞增生李斯特菌（Listeria monocytogenes）在罗非鱼皮低热加工过程中的应激适应响应。将L. monocytogenes CECT 4032在胰酪大豆肉汤培养基培养,以1.2℃/min加热速率、最终温度稳定在50℃的动态加热模式进行实验模拟训练。结果显示,当模型中理论D值与实际D值之比（f）、Z值、时间速率（a）、加热速率（E）和D值的百分比增量（c）分别为（12.96±0.79）、（4.61±0.03）℃、（0.12±0.01）min^-1、（0.51±0.01）℃/min和1.22±0.03时,Garre模型均能预测另7个微生物失活模式。以罗非鱼皮进行验证,结果发现,基于Garre模型的预测值与实测值的拟合度较高,且高于Bigelow模型,证实了L. monocytogenes在低热动态处理过程中能产生应激适应响应。结果表明,Garre模型能模拟L. monocytogenes在罗非鱼皮低热动态处理下应激适应响应,为罗非鱼皮加工和贮藏建立安全预警提供参考。更多还原

【Abstract】 In this study, the Garre model was applied to model stress adaptation response in Listeria monocytogenes inoculated on tilapia skin during dynamic low-temperature heat treatment. L. monocytogenes CECT 4032 inoculated in tryptic soy broth（TSB） was heated up to 50 ℃ with a heating rate of 1.2 ℃/min. It was found that the ratio of the theoretical D value to the actual D value（f）, Z value（Z）, time rate（a）, heating rate（E） and percentage increment of D value（c） were 12.96 ± 0.79,（4.61 ± 0.03） ℃,（0.12 ± 0.01） min^-1,（0.51 ± 0.01） ℃/min and 1.22 ± 0.03, respectively,and the model was also successfully used to predict seven other microbial inactivation patterns. Validation trials were carried out by inoculating this strain on tilapia skin, revealing that the Garre model fitted better to the experimental data than the Bigelow model. Our results demonstrate that dynamic low-temperature heat treatment can induce stress adaptation response in L. monocytogenes, which may provide a reference for constructing a security early warning system for the storage and processing of tilapia skin.更多还原