【摘要】 目的:探究抗性淀粉饮食模式下L-茶氨酸对大鼠肠道免疫功能的调节作用。方法:通过对抗性淀粉饮食模式下4周龄SD雄性大鼠进行28 d不同剂量L-茶氨酸[100,300,500 mg/（kg·d）]干预试验,考察抗性淀粉与L-茶氨酸组合营养对大鼠肠道免疫功能的调节作用。结果:在抗性淀粉饮食模式下,中、高剂量组L-茶氨酸能显著增加大鼠体重,提高回肠组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性,降低回肠丙二醛（MDA）含量;各剂量组均能显著增加回肠白介素-4（IL-4）、白介素-10（IL-10）的表达量,抑制白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的表达量,提高免疫球蛋白A（IgA）的表达水平,同时能增加各小肠肠段谷氨酸（Glu）、谷氨酰胺（Gln）含量,提高双歧杆菌属（Bifidobacterium）、普雷沃菌属9（Prevotella₉）、布劳特氏菌属（Blautia）等有益菌丰度,降低肠道有害菌幽门螺杆菌属（Helicobacter）和副萨特氏菌属（Parasutterella）占比（P<0.05）,其中,中剂量L-茶氨酸[300 mg/（kg·d）]效果最佳。结论:抗性淀粉饮食模式下L-茶氨酸对大鼠肠道免疫功能具有提高抗氧化能力、平衡促炎/抗炎因子、升高Glu与Gln含量、改善肠道菌群结构等调节作用。更多还原

【Abstract】 Objective: This study aimed to explore the effect of L-theanine on intestinal immunity of rats under resistant starch feeding. Methods: Different doses of L-theanine [100, 300, 500 mg/（kg·d）] was performed for 28 days in 4-week-old SD male rats by filling the stomach once daily under resistant starch feeding, in order to investigate the regulation of resistant starch combined with L-theanine on intestinal immunity. Results: The results were shown as follows the medium and high dose groups of L-theanine significantly increased the weight of rats, and improved the activities of ileum SOD, GSH-Px with reducing the content of MDA under resistant starch feeding; Pretreatment with L-theanine at different dosages improved the expressions of IL-4, IL-10, lowered the levels of IL-6, TNF-α, increased the level of IgA in ileum significantly. At the same time, L-theanine could heighten the contents of glutamate and glutamine in each small intestine, and improve the relative abundance of beneficial microorganisms such as Bifidobacterium, Prevotella₉, Blautia reducing the proportion of intestinal harmful microorganisms Helicobacter and Parasutterella（P<0.05）. Consequently, the medium dose group of L-theanine [300 mg/（kg·d）] combined with resistant starch had the best effect. Conclusion: Under the resistant starch feeding, L-theanine can enhance intestinal immunity of rats by improving antioxidant capacity, balancing the levels of pro-inflammatory and anti-inflammatory factors and raisng the contents of glutamate and glutamine. In addition, it can also ameliorate the structure of intestinal bacteria to affect intestinal immunity.更多还原