【摘要】 目的以三疣梭子蟹可食用部位为研究对象,探究其经过蒸煮和模拟消化后各部位镉的变化规律。方法对生鲜样本、蒸煮样本和模拟消化后样本中的总镉含量进行检测,同时优化以高效液相色谱-电感耦合等离子体质谱法（high performance liquid chromatography-inductively coupled plasma mass spectrometer,HPLC-ICP-MS）检测离子态镉(Cd²⁺)的方法,并对三疣梭子蟹可食部位Tris-HCl提取液及模拟消化液中Cd²⁺含量进行检测。结果当流动相条件为70 mmol/L草酸和133 mmol/L LiOH,pH=5.2时,分离效果最佳。该方法线性良好,相关系数r=0.999,回收率高于77.96%,相对标准偏差（relative standard deviation,RSD）为2.55%～10.28%。三疣梭子蟹经蒸煮及模拟人体消化过程后,各可食用部位的总镉含量降低;Cd²⁺含量较高的蟹膏、蟹黄部位在经过蒸煮及模拟消化后其含量下降,而肌肉中有所上升。结论三疣梭子蟹经过蒸煮后各部位中重金属镉可能发生流失或存在迁移转化,且进入人体消化过程时,不能完全溶出并被人体充分消化吸收。更多还原

【Abstract】 Objective To explore the change law of cadmium in different parts of Portunus trituberculatus after cooking and simulated digestion, taking the edible parts of Portunus trituberculatus as the research object. Methods The total cadmium content in fresh, cooked and simulated digested samples was detected, while the method for the detection of ionic cadmium(Cd²⁺) by high performance liquid chromatography-inductively coupled plasma mass spectrometry（HPLC-ICP-MS） was optimized, as well as the Tris-HCl extract from the edible part of Portunus trituberculatus and the Cd²⁺ content in the simulated digested solution. Results The optimum mobile phase conditions were 70 mmol/L oxalic acid and 133 mmol/L LiOH, pH=5.2. The linearity was good with correlation coefficient r=0.999, and the recovery was above 77.96%. The relative standard deviations（RSD） were 2.55%-10.28%. After the Portunus trituberculatus was steamed and simulated the human digestive process, the total cadmium content in each edible part was decreased. The fractions of crab paste and crab roe with higher Cd²⁺ content were decreased but increased in muscle after cooking and simulated digestion. Conclusion After cooking, heavy metal cadmium in various parts of Portunus trituberculatus may lose or migrate and transform, and when it enters the human digestion process, it cannot be completely dissolved and fully digested and absorbed by human body.更多还原