【摘要】 目的建立气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)测定韭菜中26种农药残留的方法基础上,研究惰性衬管对韭菜基质效应的消除作用。方法韭菜样品经过乙腈均质提取1.5 min,经凝胶渗透色谱法(gel permeation chromatography,GPC)净化后,进样口分别选用普通脱活衬管与超高惰性衬管,上GC-MS/MS检测。结果采用超高惰性衬管能将甲胺磷、乙酰甲胺磷、氧乐果、水胺硫磷等强极性农药的基质效应由1.58-2.51减少至1.09～1.43,拟除虫菊酯类农药基质效应由0.96-1.12减少至0.95～1.04,对26种农药在普通衬管与超高惰性衬管中响应值比值作Wilcoxon符号秩和检验,得到Z=-2.237,P=0.025,按α=0.05水准,有统计学差异。结论与普通脱活衬管相比,超高惰性衬管能更有效地消除韭菜中26种农残的基质效应,尤其显著降低甲胺磷、乙酰甲胺磷、氧乐果、水胺硫磷等强极性农药的基质效应。更多还原

【Abstract】 Objective To explore the effect of inert liner on the matrix effect based on the establishment of the method for the determination of 26 kinds of pesticide residues in leek by gas chromatography-tandem mass spectrometry(GC-MS/MS). Methods The leek sample was homogenized with acetonitrile for 1.5 min, and purified by gel permeation chromatography(GPC). The injection port was selected with ordinary deactivated liner and ultra-inert liner, and detected by GC-MS/MS. Results The ultra-high inert liner could reduce the matrix effect of strong polar pesticides such as methamidophos, acephate, omethoate, and hydrocarbophos from 1.58-2.51 to 1.09-1.43, and the matrix effect of pyrethroid pesticides was reduced from 0.96-1.12 to 0.95-1.04. The results of Wilcoxon signed rank test which performed on the ratio of the response values of 26 kinds of pesticides between ordinary deactivated liner and the ultra-high inert liner showed that Z=-2.237, P=0.025, which indicated that there was a statistical difference according to the level of α=0.05. Conclusion Compared with the ordinary deactivated liner, the ultra-high inert liner can more effectively eliminate the matrix effect of the 26 kinds of pesticide residues in leek, especially significantly reduce matrix effect of strong polar pesticides such as methamidophos, acephate, omethoate, hydrocarbophos.更多还原