【摘要】 目的建立超高效液相色谱-串联质谱法（ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS）同时测定海蛇药酒中多种成分含量的分析方法。方法采用Agilent EC-C₁₈色谱柱（4.6 mm×50 mm,1.8μm）,以水（0.1%甲酸）-乙腈（0.1%甲酸）为流动相,梯度洗脱,流速0.2 mL/min,进样量为10μL,柱温为30℃;采用电喷雾离子源（electron spray ionization,ESI）进行正离子模式监测,多反应监测模式（multiple reaction monitoring,MRM）进行定量分析。结果 1-甲基海因、紫丁香苷、绿原酸、升麻素苷、芦丁、香草酸、木犀草苷、阿魏酸、黄芩苷、槲皮素、蛇床子素分别在0.1234～6.170（r=0.9991）、0.03340～1.670（r=0.9996）、0.2578～12.89 （r=0.9998）、0.3220～16.10 （r=0.9992）、0.04975～2.488（r=0.9992）、0.02592～1.296（r=0.9991）、0.1001～5.005 （r=0.9991）、0.1054～5.270 （r=0.9994）、0.000685～0.03425 （r=0.9986）、0.00542～0.2710（r=0.9996）和0.2822～14.11 mg/L（r=0.9993）线性关系良好,平均回收率在97.4%～103.5%之间,精密度为0.34%～2.02%。结论该方法快速、专属性强、灵敏度高,可用于海蛇药酒的质量控制。更多还原

【Abstract】 Objective To establish a method for simultaneous determination of 11 kinds of main active components in seasnake medicinal liquor by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. Methods UPLC-MS/MS analysis was performed on a Agilent EC-C₁₈（4.6 mm×50 mm, 1.8 μm） eluted with acetonitrile and ultrapure water both containing 0.1% formic acid in a gradient program. The flow rate was 0.2 mL/min, the injection volume was 10 μL at column temperature 30 ℃. The multiple-reaction monitoring（MRM） scanning mode was employed for quantification with switching electrospray ion source polarity in positive mode. Results 1-Methylhydantoin, ligustrin, chlorogenic acid, prim-O-glucosylcimifugin, rutin, vanillic acid, luteoloside, ferulic acid, baicalin, quercetin, osthole showed a good linearity in the ranges of 0.1234-6.170（r=0.9991）, 0.03340-1.670（r=0.9996）, 0.2578-12.89（r=0.9998）, 0.3220-16.10（r=0.9992）, 0.04975-2.488（r=0.9992）, 0.02592-1.296（r=0.9991）, 0.1001-5.005（r=0.9991）, 0.1054-5.270（r=0.9994）, 0.000685-0.03425（r=0.9986）, 0.00542-0.2710（r=0.9996） and 0.2822-14.11 mg/L（r=0.9993）. The average recoveries varied from 97.4%-103.5%. The RSD values of precision varied from 0.34%-2.02%. Conclusions The method is rapid, specific and sensitive, and can be applied in the quality control of seasnake medicinal liquor.更多还原