【摘要】 目的建立基质分离固相萃取结合液相色谱串联质谱法（liquidchromatographytandemmass spectrometry, LC-MS/MS）快速测定牛肉中克伦特罗、莱克多巴胺、沙丁胺醇、特布他林等4种β₂-受体激动剂类兽药残留。方法样品经葡萄糖醛苷酶/硫酸酯酶酶解、盐析后经CNW兽药定量检测分散固相萃取纯化包净化。采用LC-MS/MS电喷雾正离子模式、多反应监测采集（multiple reaction monitoring acquisition, MRM）测定。结果当4种β₂-受体激动剂类兽药化合物空白样本加标水平在2.5、12.5、50mg/kg时,回收率为95.39%～106.59%,相对标准偏差（relative standard deviation, RSD）为0.98%～13.26%。4种目标化合物的检出限为0.1～0.3mg/kg,定量限为0.3～1.0mg/kg。针对市售66件牛肉样品中目标化合物进行检测,结果均未检出。结论与传统通过性固相萃取法相比,兽药定量检测分散固相萃取纯化法净化效果更好,基质效应更低。CNW兽药定量检测分散固相萃取法可用于牛肉中克伦特罗、莱克多巴胺、沙丁胺醇、特布他林4种β₂-受体激动剂类兽药残留快速检测。更多还原

【Abstract】 Objective To a establish a method for the rapid determination of 4 β₂-agonist veterinary drug residues in beef, including clenbuterol, ractopamine, salbutamol and terbutaline by matrix separation solid phase extraction combined with liquid chromatography-tandem mass spectrometry（LC-MS/MS）. Methods The samples were hydrolyzed by gluconidase/sulfatase, salted out, purified by CNW veterinary drug quantitative detection and dispersive solid phase extraction purification method, and determined by LC-MS/MS electrospray positive ion mode and multiple reaction monitoring acquisition（MRM）. Results When the standard levels of 4 kinds of β₂-agonist veterinary drug compounds in blank samples were spiked at 2.5, 12.5, and 50 mg/kg, the recoveries were 95.39%-106.46%, and the relative standard deviation were 0.98%-13.26%. The limits of detection of the 4 kinds of target compounds were 0.1-0.3 mg/kg, and the limits of quantification were 0.3-1.0 mg/kg. The target compounds were detected in 66 beef samples on the market, but the results were not detected. Conclusion Compared with the traditional solid-phase extraction method, quantitative determination of veterinary drugs dispersive solid phase extraction purification method has better purification effect and lower matrix effect. CNW veterinary drug quantitative detection dispersive solid-phase extraction method can be used for rapid detection of 4 kinds of β₂-agonist veterinary drug residues clenbuterol, ractopamine, salbutamol, and terbutaline in beef.更多还原