【摘要】 目的建立通过式固相萃取柱结合前处理技术-液相色谱串联质谱法快速测定熟肉制品中克伦特罗、莱克多巴胺、沙丁胺醇、特布他林4种常见β₂-受体激动剂类兽药残留的分析方法。方法熟肉制品采用5%三氯乙酸酸解, NaCl盐析乙腈萃取,正己烷除脂后经通过式固相萃取柱和QuEChERS净化,超高效液相色谱-串联质谱于电喷雾正离子源（ESI+）和多反应监测扫描模式下进行测定,基质匹配标准曲线外标法定量。结果 4种目标化合物阴性加标水平在2、5、10mg/kg下,回收率在77.2%～104.7%之间,相对标准偏差在2.9%～9.6%之间。4种目标化合物的检出限在0.2～0.6mg/kg之间,定量限在0.6～2.0mg/kg之间。对30件熟肉样品进行目标化合物的检测,未检测到β₂-受体激动剂类兽药残留。结论该方法操作简便、快速,灵敏度和准确度高,包含种类较宽,具有一定的拓展性,适用熟肉制品相关残留的日常监测。更多还原

【Abstract】 Objective To establish a method for the rapid determination of veterinary drug residues of 4 common β₂-receptor agonists in cooked meat products, including clenbuterol, ractopamine, salbutamol, and terbutaline, by pass-through solid-phase extraction column combined with pretreatment technique-liquid chromatography tandem mass spectrometry. Methods The cooked meat products were subjected to acidolysis with 5% trichloroacetic acid, extraction with NaCl salting-out acetonitrile, and degreased with n-hexane, which were then purified by passing through a solid-phase extraction column and QuEChERS. The ultra-performance liquid chromatography-tandem mass spectrometry was performed in electrospray positive ion source（ESI+） and multi-reaction monitoring scanning mode. The matrix matching standard curve was quantified by external standard method. Results The recoveries of the 4 target compounds were 77.2%-104.7% and the relative standard deviation was 2.9%-9.6% at the negative spiked levels of 2, 5, and 10 mg/kg, respectively. The detection limits of the 4 target compounds were 0.2-0.6 mg/kg, and the quantitative limits were 0.6-2.0 mg/kg. Thirty cooked meat samples were tested for the target compound and no residue of β₂-receptor agonist veterinary drug was detected. Conclusion This method is simple, rapid, sensitive and accurate, has a wide range of types, and has certain expansibility. It is suitable for the daily monitoring of related residues of cooked meat products.更多还原