【摘要】 目的探究力学微环境对脂肪来源干细胞(adipose-derived stem cells, ADSCs)生物学功能的影响,为干细胞的基础研究及临床应用提供新思路。方法自2021年3—7月,南方医科大学南方医院整形外科于体外在不同刚度培养板中培养ADSCs,进行细胞划痕实验,诱导成脂,检测RNA和蛋白质的表达情况,对比不同刚度下ADSCs的迁移能力和成脂分化功能。结果培养板刚度越高,ADSCs的迁移能力越强;培养板刚度为4 kPa时,ADSCs成脂分化能力比2 kPa和6 kPa更强。结论 ADSCs的迁移能力与刚度成正相关,刚度在4 kPa时能够有效促进ADSCs的成脂分化。更多还原

【Abstract】 Objective To explore the influence of mechanical microenvironment on the biological functions of adipose-derived stem cells(ADSCs) and to provide new ideas for the basic research and clinical application of stem cells. Methods From March to July2021, the ADSCs were cultured in different stiffness plates in vitro, scratch assay and adipogenic differentiation were performed. The migration and adipogenic differentiation of ADSCs under different stiffness was compared by detection of expression of RNA and proteins.Results The higher the stiffness of the culture plate, the stronger the migration ability of ADSCs. When the stiffness of the culture plate was 4 kPa, the adipogenic differentiation capacity of ADSCs was stronger than that of 2 kPa and 6 kPa. Conclusion The migration ability of ADSCs was positively related to stiffness. The stiffness of 4 kPa can effectively promote the adipogenic differentiation of ADSCs.更多还原