【摘要】 目的观察药物难治性癫痫患儿的肠道菌群变化,明确肠道菌群与药物难治性癫痫的关系。方法初诊癫痫患儿(癫痫组)根据抗癫痫治疗(6月)效果分为药物难治(癫痫发作减少≤50%)组和药物反应(癫痫发作减少>50%)组,另选25例健康儿童作为对照组。采集癫痫患儿治疗前后及健康儿童的粪便标本,通过16 s核糖体DNA高通量测序分析肠道菌群组成。结果治疗前,癫痫组与对照组肠道菌群多样性比较,P均<0.05;药物难治组与药物反应组肠道菌群多样性比较,P均<0.05。药物难治组治疗前的厚壁菌门与拟杆菌门的比例高于药物反应组(P均<0.05)。治疗前,药物难治组患儿与对照组、药物反应组患儿肠道菌属比较,梭菌属和克罗诺杆菌丰度增多,拟杆菌属和双歧杆菌属丰度减少(P均<0.05)。结论药物难治性癫痫患儿存在肠道菌群失调,多样性及稀有性肠道菌群增多。更多还原

【Abstract】 Objective To observe the changes of gut microbiota in children with drug-resistant epilepsy and to illustrate the relationship between intestinal flora and drug-resistant epilepsy.Methods Children with newly diagnosed epilepsy were grouped into the drug-resistant(seizure reduction≤50%) group and drug-responsive(seizure reduction> 50%)group according to the effect of anti-epileptic treatment(6 months),and meanwhile,another 25 healthy children were selected as the control group.Stool specimens were collected from all children with epilepsy before and after treatment and from children in the healthy control group,and the microbial composition was analyzed by 16 s ribosomal DNA highthroughput sequencing.Results There was a statistically significant difference in the diversity of gut microbiota between the epilepsy group and the control group(P<0.05).There was a statistically significant difference in the diversity of gut microbiota between the drug-resistant group and the drug-responsive group(P<0.05).The ratio of Firmicutes to Bacteroides in the drug-resistant group before treatment was significantly higher than those of the other groups(P<0.05).Before treatment,we compared the abundance of intestinal bacteria in children between the drug-resistant group and the control group and the drug-responsive group,the abundance of Clostridium and Cronobacter increased,and the abundance of Bacteroides and Bifidobacterium decreased(all P<0.05).Conclusion Children with drug-resistant epilepsy have intestinal flora imbalance,with increased diversity of intestinal flora and increased rare intestinal flora.更多还原