【摘要】 苹果黑腐皮壳菌(Valsa mali var. mali)引起的苹果树腐烂病是我国苹果产区发生严重的病害之一,了解病原菌侵染寄主不同时期的基因表达有助于揭示病菌的致病机制和苹果抗病机制。本实验应用Illumina平台对苹果黑腐皮壳菌侵染不同时期的寄主枝干发病过程进行转录组测序,同时通过与未侵染的病原菌和寄主组织进行比较。在病原菌侵染过程中,病原菌中发现4092个差异表达基因,寄主中发现16966个差异基因,通过对三个时期的上调差异表达基因进行GO和KEGG分析,结果表明病原菌侵染导致了寄主细胞壁降解、毒素物质合成、寄主抗毒物质分解和自身营养调节等过程;寄主主要通过膜脂过氧化作用、活性氧清除酶和防御酶抵抗病原菌的入侵。上述研究明确了苹果黑腐皮壳菌与寄主互作的生物学过程,为探讨病菌与寄主的分子互作机制奠定基础。更多还原

【Abstract】 Apple Valsa canker caused by Valsa mali is one of the most serious diseases in apple producing areas in China.Understanding the gene expression of pathogen infected host at different periods is helpful to find out pathogenic mechanism of the pathogen infection and resisting mechanism of host plant. This paper mainly using Illumina platform to sequence the transcriptome of pathogen and host during infection of V. mali, and compare with the uninfected pathogen and host, 4092 differential expressed genes from pathogen and 16966 differential expressed genes from host were obtained. Go and KEGG analysis of the up-regulated differential expressed genes in the three periods were carried out respectively. The results showed that degradation of host cell wall, synthesis of toxin, decomposition of anti toxin and self nutrition regulation during the pathogen infection process, and the host mainly resisted the invasion of pathogen through membrane lipid peroxidation,active oxygen scavenging enzyme and defense enzyme. All results above explained the biological process of interaction between the V. mali and host, and provided a basis for probing molecular interaction mechanism between pathogen and apple.更多还原