【摘要】 【目的】探讨黄芪三七药对对大鼠脑缺血再灌注后炎症级联反应的抑制作用及神经保护作用。【方法】将50只SD大鼠随机分为假手术组、模型组、黄芪组、三七组、黄芪三七组,每组10只。假手术组仅行游离血管操作,不做插线处理,其他各组采用改良Longa法构建大脑中动脉闭塞(MCAO)大鼠模型,缺血2 h,再灌注24 h后,进行神经功能缺损评分,苏木素-伊红(HE)染色观察大鼠脑组织病理改变,原位末端脱氧核苷酸转移酶(TdT)标记(TUNEL)染色法测定脑组织神经元细胞的凋亡情况,免疫组织化学法检测脑组织核转录因子κB p65(NF-κB p65)、肿瘤坏死因子α(TNF-α)、基质金属蛋白酶9(MMP-9)的表达。【结果】与假手术组比较,模型组大鼠神经功能缺损评分升高,脑组织可见明显的病理损伤,神经元坏死率、神经元凋亡率增加,脑组织NF-κB p65、TNF-α、MMP-9的表达水平均显著升高(P<0.05);与模型组比较,黄芪组、三七组、黄芪三七组神经功能缺损评分均降低,脑组织损伤不同程度得到改善,神经元坏死率、神经元凋亡率均减小,脑组织NF-κB p65、TNF-α、MMP-9的表达水平均显著降低,其中黄芪三七组的差异均有统计学意义(P<0.05或P<0.01)。【结论】黄芪三七药对可有效减轻大鼠脑缺血再灌注损伤,其神经保护作用机制可能与抑制炎症级联反应,减少神经元的凋亡有关,且疗效优于黄芪、三七单药。更多还原

【Abstract】 Objective To explore the inhibitory effect of herbal pair of Radix Astragali and Radix Notoginseng on inflammatory cascade reaction after cerebral ischemia-reperfusion injury in rats and the neuroprotective effect.Methods Fifty SD rats were randomly divided into 5 groups,namely sham-operation group,model group,Radix Astragali group,Radix Notoginseng group and Radix Astragali-Radix Notoginseng group,10 rats in each group.The rats in the sham-operation group were given only isolating middle cerebral artery,and no inserting thread operation,and the rats in the other groups were induced into middle cerebral artery occlusion(MCAO) model by Longa method.After ischemia for 2 h and reperfusion for 24 h,the neurological deficit scores were calculated,pathological features of brain tissues were observed by haematoxylin-eosin staining,and apoptosis of nerve cells in brain tissues was measured by terminal deoxyribonucleotidyl transferse(TdT)-mediated biotin-16-dUTP nick-end labelling(TUNEL) assay,and expression levels of nuclear factor-κB(NF-κB) p65,tumor necrosis factor a(TNF-α),and matrix metalloproteinase 9(MMP-9) in brain tissues were detected by immunohistochemical method.Results Compared with the sham-operation group,the scores of neurological deficit were increased,obvious pathological damage in brain tissues was seen,apoptosis rate of neurons and apoptosis rate of neurons in brain tissues were enhanced,and expression levels of NF-κB p65,TNF-α and MMP-9 in brain tissues were raised in the model group(P <0.05).Compared with the model group,the scores of neurological deficit were decreased,pathological damage in brain tissues was improved to varying degrees,apoptosis rate of neurons and apoptosis rate of neurons in brain tissues were lowered,and expression levels of NF-κB p65,TNF-α and MMP-9 in brain tissues were reduced in the Radix Astragali group,Radix Notoginseng group and Radix Astragali-Radix Notoginseng group,of which the differences were significant in the Radix Astragali-Radix Notoginseng group(P <0.05 or P <0.01).Conclusion Radix Astragali-Radix Notoginseng pair is effective for alleviating the cerebral ischemia reperfusion injury in rats,and its neuroprotective mechanism may be related to inhibiting inflammatory cascade reaction and reducing neuron apoptosis,the improvement effect being superior to Radix Astragali or Radix Notoginsengalone.更多还原